Improving endothelial cell adhesion to vascular graft surfaces: clinical need and strategies.

Improving endothelial cell adhesion to vascular graft surfaces: clinical need and strategies. Research Abstract Details 

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Improving endothelial cell adhesion to vascular graft surfaces: clinical need and strategies. Abstract Text:

V D Bhat,B Klitzman,K Koger,G A Truskey,W M Reichert,

Synthetic vascular grafts do not spontaneously endothelialize in humans and require some form of anticoagulation to maintain patency. Preseeding synthetic graft materials such as expanded polytetrafluoroethylene (ePTFE) and polyethylene terephthalate (PET) with endothelial cells (EC) has been examined in various in vitro and in vivo models. Although various studies provide encouraging results, clinical trials for EC seeding on synthetic grafts have not been equally successful. This paper provides a brief review of the various reports on EC seeding in animal and clinical studies. We discuss the inefficiencies associated with the EC seeding process and examine plasma protein treatment of the graft surfaces as a viable option for improving EC attachment, retention and spreading. As an alternative to existing therapies we present data on a heterogeneous ligand treatment of fibronectin (Fn) and avidin-biotin for enhanced human umbilical vein endothelial cell (HUVEC) adhesion to ePTFE graft surfaces. Control consisted of HUVECs seeded on Fn treated ePTFE graft surfaces. Functionality of HUVECs was assessed by measuring prostacyclin production of cells on both homogeneous and heterogeneous ligand treated surfaces. Laminar flow studies with a variable width flow chamber and scanning electron microscopy were used to measure initial cell retention and observe initial cell spreading on ePTFE surfaces, respectively. HUVEC retention on heterogeneous ligand treated graft surface was significantly (p < 0.001) higher compared to homogeneous ligand treated surfaces for shear stress in the range of 10-30 dyn cm(-2). HUVEC showed more cellular spreading on the heterogeneous ligand treated surface after seeding for 1-2 h. In vivo experimentation was performed in immune deficient (nude) rats by replacing a section of both the femoral arteries with 8 mnm long, 1 mm internal diameter denucleated ePTFE grafts treated with homogeneous and heterogeneous ligands respectively. Both grafts were seeded with similar cell density for 15 min prior to implantation. EC attachment and retention was measured by staining EC with hematoxylin and counting the cells before and after flow using light microscopy. The results indicate that a heterogeneous ligand treatment of graft surfaces using avidin-biotin and Fn-integrin attachment mechanisms increase cell seeding efficiency, initial cell retention and cellular spreading.

Improving endothelial cell adhesion to vascular graft surfaces: clinical need and strategies. Publishing Authors By Initials

VD Bhat,B Klitzman,K Koger,GA Truskey,WM Reichert,

For similar cardiovascular system: blood vessels: veins: portal system: umbilical veins research abstracts see: cardiovascular system: blood vessels: veins: portal system: umbilical veins research

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MEDLINE DATE:

Improving endothelial cell adhesion to vascular graft surfaces: clinical need and strategies. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of biomaterials science. Polymer edition

VOLUME: 9

Page Numbers: 1117-35

Journal Abbreviation:

ISSN: 0920-5063

DAY: 20

MONTH: 02

YEAR: 1998

Improving endothelial cell adhesion to vascular graft surfaces: clinical need and strategies. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9007393

Improving endothelial cell adhesion to vascular graft surfaces: clinical need and strategies. Keywords Mesh Terms:

KEYWORDS: Umbilical Veins

MESH TERMS: cytology

Chemical & Substance for Abstract: Improving endothelial cell adhesion to vascular graft surfaces: clinical need and strategies. Information

Substance Name: Polytetrafluoroethylene

Registry Number: 9002-84-0

Grant and Affiliation Information for Improving endothelial cell adhesion to vascular graft surfaces: clinical need and strategies.

AFFILIATION: Department of Biomedical Engineering & Centre for Cellular and Biosurface Engineering, Duke University, Durham, NC 27708-0281, USA.

Country: NETHERLANDS

AGENCY: United States NHLBI

GRANT: HL-44972

ACRONYM: HL

MEDLINETA: J Biomater Sci Polym Ed

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