Improvement of microemulsion electrokinetic chromatography for measuring octanol-water partition coefficients.

Improvement of microemulsion electrokinetic chromatography for measuring octanol-water partition coefficients. Research Abstract Details 

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Improvement of microemulsion electrokinetic chromatography for measuring octanol-water partition coefficients. Abstract Text:

Zhining Xia,Xuemei Jiang,Xiaojing Mu,Hua Chen,

Microemulsion electrokinetic chromatography (MEEKC) has been used to indirectly measure octanol-water partition coefficients (log P(ow)) of compounds. In order to obtain an accurate log P(ow) value, the electrophoretic mobilities of the microemulsion phase (mu(me)) and the analyte (mu(eff)) in MEEKC must be accurately required. However, in conventional MEEKC, the shortage of obtaining mu(me) with a tracing method was discovered, and the influences of concentration, injection volume of analyte, and high electric field on measuring mu(eff) were also found. In this paper, a novel method called improved MEEKC (I-MEEKC) was developed to avoid the problems mentioned above. In I-MEEKC, a nonlinearity fitting program was used to obtain mu(me) to avoid the error from tracing mu(me); the extrapolating method was used to eliminate the effects of concentrations and injection volumes of analytes on mu(eff) measurement, and an enough stable microemulsion was selected to eliminate the effect of high electric field on mu(eff )measurement. Then the novel method was applied to estimate log P(ow) of uncharged compounds and charged pharmaceuticals compared to the conventional MEEKC. The log P(ow) of all analytes obtained by I-MEEKC agreed with those obtained by classical shake flask or literature values, the errors between them were within 0.1 logarithm units, better than the ones by conventional MEEKC.

Improvement of microemulsion electrokinetic chromatography for measuring octanol-water partition coefficients. Publishing Authors By Initials

Z Xia,X Jiang,X Mu,H Chen,

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Improvement of microemulsion electrokinetic chromatography for measuring octanol-water partition coefficients. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Electrophoresis

VOLUME: 29

Page Numbers: 835-42

Journal Abbreviation: Electrophoresis

ISSN: 0173-0835

DAY: 28

MONTH: Feb

YEAR: 2008

Improvement of microemulsion electrokinetic chromatography for measuring octanol-water partition coefficients. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Improvement of microemulsion electrokinetic chromatography for measuring octanol-water partition coefficients.

AFFILIATION: Department of Pharmaceutics, College of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R. China.

Country: Germany

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MEDLINETA: Electrophoresis

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