Improved method to raise polyclonal antibody using enhanced green fluorescent protein transgenic mice.

Improved method to raise polyclonal antibody using enhanced green fluorescent protein transgenic mice. Research Abstract Details 

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Improved method to raise polyclonal antibody using enhanced green fluorescent protein transgenic mice. Abstract Text:

Jianke Ren,Long Wang,Guoxiang Liu,Wen Zhang,Zhejin Sheng,Zhugang Wang,Jian Fei,Jianke Ren,Long Wang,Guoxiang Liu,Wen Zhang,Zhejin Sheng,Zhugang Wang,Jian Fei,

Recombinant fusion protein is widely used as an antigen to raise antibodies against the epitope of a target protein. However, the concomitant anticarrier antibody in resulting antiserum reduces the production of the desired antibody and brings about unwanted non-specific immune reactions. It is proposed that the carrier protein transgenic animal could be used to solve this problem. To validate this hypothesis, enhanced green fluorescent protein (EGFP) transgenic mice were produced. By immunizing the mice with fusion protein His6HAtag-EGFP, we showed that the antiserum from the transgenic mice had higher titer antibody against His6HA tag and lower titer antibody against EGFP compared with that from wild-type mice. Therefore, this report describes an improved method to raise high titer antipeptide polyclonal antibody using EGFP transgenic mice that could have application potential in antibody preparation.

Improved method to raise polyclonal antibody using enhanced green fluorescent protein transgenic mice. Publishing Authors By Initials

J Ren,L Wang,G Liu,W Zhang,Z Sheng,Z Wang,J Fei,J Ren,L Wang,G Liu,W Zhang,Z Sheng,Z Wang,J Fei,

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Improved method to raise polyclonal antibody using enhanced green fluorescent protein transgenic mice. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Acta biochimica et biophysica Sinica

VOLUME: 40

Page Numbers: 111-5

Journal Abbreviation: Acta Biochim. Biophys. Sin. (S

ISSN: 1745-7270

DAY: 31

MONTH: Feb

YEAR: 2008

Improved method to raise polyclonal antibody using enhanced green fluorescent protein transgenic mice. Information

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LANGUAGE: eng

NlmUniqueID: 101206716

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AFFILIATION: Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. jfei@mail.tongji.edu.cn.

Country: China

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MEDLINETA: Acta Biochim Biophys Sin (Shan

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