Improved expression of fusion protein using a cysteine- protease- and chitinase-deficient Bombyx mori (silkworm) multiple nucleopolyhedrovirus bacmid in silkworm larvae.

Improved expression of fusion protein using a cysteine- protease- and chitinase-deficient Bombyx mori (silkworm) multiple nucleopolyhedrovirus bacmid in silkworm larvae. Research Abstract Details 

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Improved expression of fusion protein using a cysteine- protease- and chitinase-deficient Bombyx mori (silkworm) multiple nucleopolyhedrovirus bacmid in silkworm larvae. Abstract Text:

Enoch Y Park,Takahiro Abe,Tatsuya Kato,Enoch Y Park,Takahiro Abe,Tatsuya Kato,

A bacmid system of BmMNPV [Bombyx mori (silkworm) multiple nucleopolyhedrovirus] with both the cysteine-protease and chitinase genes deleted (BmMNPV-CP(-)-Chi(-)) was constructed. The viral protease and chitinase activities in the haemolymph of Bombyx mori larvae infected with this BmMNPV-CP(-)-Chi(-) bacmid were reduced by 95 and 50% respectively. By using this system, a GFP(uv)-beta3GnT2 (green fluorescent protein excited by UV light-beta1,3-N-acetylglucosaminyltransferase 2) fusion protein was successfully expressed in silkworm larvae with less protein degradation and without larvae liquefaction; beta3GnT2 activity improved 2.8-fold over that of unmodified bacmid. This BmMNPV-CP(-)-Chi(-) bacmid system provides rapid protein production in silkworms and can be used for the production of recombinant eukaryotic proteins without proteolytic degradation.

Improved expression of fusion protein using a cysteine- protease- and chitinase-deficient Bombyx mori (silkworm) multiple nucleopolyhedrovirus bacmid in silkworm larvae. Publishing Authors By Initials

EY Park,T Abe,T Kato,EY Park,T Abe,T Kato,

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Improved expression of fusion protein using a cysteine- protease- and chitinase-deficient Bombyx mori (silkworm) multiple nucleopolyhedrovirus bacmid in silkworm larvae. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biotechnology and applied biochemistry

VOLUME: 49

Page Numbers: 135-40

Journal Abbreviation: Biotechnol. Appl. Biochem.

ISSN: 1470-8744

DAY: 15

MONTH: Feb

YEAR: 2008

Improved expression of fusion protein using a cysteine- protease- and chitinase-deficient Bombyx mori (silkworm) multiple nucleopolyhedrovirus bacmid in silkworm larvae. Information

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LANGUAGE: eng

NlmUniqueID: 8609465

Improved expression of fusion protein using a cysteine- protease- and chitinase-deficient Bombyx mori (silkworm) multiple nucleopolyhedrovirus bacmid in silkworm larvae. Keywords Mesh Terms:

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Grant and Affiliation Information for Improved expression of fusion protein using a cysteine- protease- and chitinase-deficient Bombyx mori (silkworm) multiple nucleopolyhedrovirus bacmid in silkworm larvae.

AFFILIATION: Laboratory of Biotechnology, Integrated Bioscience Section, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. yspark@agr.shizuoka.ac.jp

Country: England

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MEDLINETA: Biotechnol Appl Biochem

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