Improved expression of a soluble single chain antibody fusion protein containing tumor necrosis factor in Escherichia coli.

Improved expression of a soluble single chain antibody fusion protein containing tumor necrosis factor in Escherichia coli. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Improved expression of a soluble single chain antibody fusion protein containing tumor necrosis factor in Escherichia coli. Abstract Text:

Sehoon Kim,Lawrence H Cheung,Weihe Zhang,Michael G Rosenblum,Sehoon Kim,Lawrence H Cheung,Weihe Zhang,Michael G Rosenblum,Sehoon Kim,Lawrence H Cheung,Weihe Zhang,Michael G Rosenblum,

The immunocytokine scFvMEL/TNF is a fusion protein composed of tumor necrosis factor (TNF-alpha) and a single-chain Fv antibody scFvMEL targeting the melanoma-associated gp240 antigen. The fusion protein containing thioredoxin and a hexa-histidine tag was expressed in two Escherichia coli host cells, AD494 (DE3) pLysS and T7 Express I (q). The cell growth and expression level of target protein, His-tagged scFvMEL/TNF, were highly dependent on the induction temperature, inducer types and host strains. The ratio of insoluble to soluble target proteins was found to be controllable and could be minimized using cold shock conditions at less than 18 degrees C. The total productivity of soluble target protein was further improved by high cell density cultivation using a DO-STAT feeding strategy. The scFvMEL/TNF purified under their conditions was specifically cytotoxic to gp240-antigen positive melanoma A375-M cells as previously described.

Improved expression of a soluble single chain antibody fusion protein containing tumor necrosis factor in Escherichia coli. Publishing Authors By Initials

S Kim,LH Cheung,W Zhang,MG Rosenblum,S Kim,LH Cheung,W Zhang,MG Rosenblum,S Kim,LH Cheung,W Zhang,MG Rosenblum,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Improved expression of a soluble single chain antibody fusion protein containing tumor necrosis factor in Escherichia coli. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Applied microbiology and biotechnology

VOLUME: 77

Page Numbers: 99-106

Journal Abbreviation: Appl. Microbiol. Biotechnol.

ISSN: 0175-7598

DAY: 17

MONTH: 08

YEAR: 2007

Improved expression of a soluble single chain antibody fusion protein containing tumor necrosis factor in Escherichia coli. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8406612

Improved expression of a soluble single chain antibody fusion protein containing tumor necrosis factor in Escherichia coli. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Improved expression of a soluble single chain antibody fusion protein containing tumor necrosis factor in Escherichia coli. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Improved expression of a soluble single chain antibody fusion protein containing tumor necrosis factor in Escherichia coli.

AFFILIATION: Department of Experimental Therapeutics, The University of Texas, M.D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030, USA.

Country: Germany

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Appl Microbiol Biotechnol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Improved expression of a soluble single chain antibody fusion protein containing tumor necrosis factor in Escherichia coli Related Publications

• BMP receptor ALK3 controls collecting system development.
• BMP-2 and OP-1 exert direct and opposite effects on renal branching morphogenesis.
• BMP7 controls collecting tubule cell proliferation and apoptosis via Smad1-dependent and -independent pathways.
• Balloon dilatation of biliary-enteric strictures in children.
• Cancer in infancy and childhood.
• Chorea (Sydenham) a study of 58 additional patients.
• Correlates of drug treatment program staff's self efficacy to support their clients' hepatitis C virus (HCV) related needs.
• Extramedullary plasmacytoma manifested as an epidural mass.
• FATTY ACID ANALYSIS OF TWO EXPERIMENTAL TRANSMISSIBLE GLIAL TUMORS BY GAS-LIQUID CHROMATOGRAPHY.
• Hepatitis C knowledge among staff in U.S. drug treatment programs.
• Improved expression of a soluble single chain antibody fusion protein containing tumor necrosis factor in Escherichia coli.
• Improving the emergence profile of an anterior implant restoration using a custom healing cap and a "double cast" abutment.
• Induction of peripheral tolerance by local delivery of dendritic cell progenitors to cardiac allografts in a murine heterotopic heart transplantation model.
• MR imaging of migraine.
• MR imaging of patients with migraine.
• MR of central nervous system actinomycosis.
• Neurological manifestations of the acquired immunodeficiency syndrome (AIDS): experience at UCSF and review of the literature. 1985.
• Percutaneous balloon catheter dilatation of benign ureteral strictures: effect of multiple dilatation procedures on long-term patency.
• Percutaneous biliary drainage in patients with nondilated intrahepatic bile ducts.
• Pharmacodynamics of phosphonomycin after oral administration in man.
• Primary amyloidoma of the brain: CT and MR presentation.
• Radiologic placement of long-term hemodialysis catheters in occluded jugular or subclavian veins or through patent thyrocervical collateral veins.
• Recent progress in alpha-ray spectroscopy.
• Renal actinomycosis; a case report.
• Synthesis and activity of a potent, specific azabicyclo[3.3.0]-octane-based DPP II inhibitor.
• The Vocabulary of Health.
• The immunocytokine scFv23/TNF targeting HER-2/neu induces synergistic cytotoxic effects with 5-fluorouracil in TNF-resistant pancreatic cancer cell lines.
• Three-dimensional CT for pre- and postsurgical imaging of patients with craniosynostosis: correlation of operative procedure and radiologic imaging.
• Typhoid and paratyphoid fever in immunized subjects.
• [Desire for a child: a mad passion?]