Improved cytosolic translocation and tumor-killing activity of Tat-shepherdin conjugates mediated by co-treatment with Tat-fused endosome-disruptive HA2 peptide.

Improved cytosolic translocation and tumor-killing activity of Tat-shepherdin conjugates mediated by co-treatment with Tat-fused endosome-disruptive HA2 peptide. Research Abstract Details 

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Improved cytosolic translocation and tumor-killing activity of Tat-shepherdin conjugates mediated by co-treatment with Tat-fused endosome-disruptive HA2 peptide. Abstract Text:

Toshiki Sugita,Tomoaki Yoshikawa,Yohei Mukai,Natsue Yamanada,Sunao Imai,Kazuya Nagano,Yasunobu Yoshida,Hiroko Shibata,Yasuo Yoshioka,Shinsaku Nakagawa,Haruhiko Kamada,Shin-ichi Tsunoda,Yasuo Tsutsumi,Toshiki Sugita,Tomoaki Yoshikawa,Yohei Mukai,Natsue Yamanada,Sunao Imai,Kazuya Nagano,Yasunobu Yoshida,Hiroko Shibata,Yasuo Yoshioka,Shinsaku Nakagawa,Haruhiko Kamada,Shin-ichi Tsunoda,Yasuo Tsutsumi,

Tat peptides are useful carriers for delivering biologic molecules into the cell for both functional analysis of intracellular disease-related proteins and treatment of refractory diseases. Most internalized Tat-fused cargos (Tat-cargos) are trapped within the endosome, however, which limits the biologic function of the cargo. In this study, we demonstrated that Tat-fused HA2 peptide (HA2Tat), an endosome disrupted peptide, enhanced the endosome-escape efficiency of Tat-cargos. In cells treated with a mixture of fluorescein isothiocyanate-labeled Tat and HA2Tat, widespread fluorescence was observed throughout the cytosol. In addition, this HA2Tat-mediated cytosolic delivery technique led to enhanced cytotoxicity of Tat-fused anti-cancer peptides, specifically shepherdin. Thus, we improved the function of the delivered molecules by co-treating with HA2Tat and propose that this is a useful method for the delivery of therapeutic macromolecules into the cytosol.

Improved cytosolic translocation and tumor-killing activity of Tat-shepherdin conjugates mediated by co-treatment with Tat-fused endosome-disruptive HA2 peptide. Publishing Authors By Initials

T Sugita,T Yoshikawa,Y Mukai,N Yamanada,S Imai,K Nagano,Y Yoshida,H Shibata,Y Yoshioka,S Nakagawa,H Kamada,S Tsunoda,Y Tsutsumi,T Sugita,T Yoshikawa,Y Mukai,N Yamanada,S Imai,K Nagano,Y Yoshida,H Shibata,Y Yoshioka,S Nakagawa,H Kamada,S Tsunoda,Y Tsutsumi,

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Improved cytosolic translocation and tumor-killing activity of Tat-shepherdin conjugates mediated by co-treatment with Tat-fused endosome-disruptive HA2 peptide. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochemical and biophysical research communication

VOLUME: 363

Page Numbers: 1027-32

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 1090-2104

DAY: 29

MONTH: 09

YEAR: 2007

Improved cytosolic translocation and tumor-killing activity of Tat-shepherdin conjugates mediated by co-treatment with Tat-fused endosome-disruptive HA2 peptide. Information

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LANGUAGE: eng

NlmUniqueID: 372516

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Grant and Affiliation Information for Improved cytosolic translocation and tumor-killing activity of Tat-shepherdin conjugates mediated by co-treatment with Tat-fused endosome-disruptive HA2 peptide.

AFFILIATION: Laboratory of Pharmaceutical Proteomics, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.

Country: United States

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MEDLINETA: Biochem Biophys Res Commun

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