Improved contamination control for a rapid phage-based rifampicin resistance test for Mycobacterium tuberculosis.

Improved contamination control for a rapid phage-based rifampicin resistance test for Mycobacterium tuberculosis. Research Abstract Details 

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Improved contamination control for a rapid phage-based rifampicin resistance test for Mycobacterium tuberculosis. Abstract Text:

Richard Mole,Andre Trollip,Celeste Abrahams,Marlein Bosman,Heidi Albert,Richard Mole,Andre Trollip,Celeste Abrahams,Marlein Bosman,Heidi Albert,

A prospective study was conducted of the rapid FASTPlaque-Response test for determination of rifampicin resistance in Mycobacterium tuberculosis with and without the addition of an antimicrobial supplement containing nystatin, oxacillin and aztreonam (NOA) to control specimen-related contamination. A total of 631 smear-positive sputum specimens was tested. The age of specimens ranged from 0 to 21 days. The NOA antimicrobial was effective at controlling contamination, with 4.1 % of specimens contaminated when the NOA antimicrobial supplement was used compared with 13.9 % contamination without NOA. Overall levels of interpretability of the test with NOA were 87.8 % with specimens of < or =3 days and 79.0 % for all specimens. This compared with 70.1 and 73.8 % readable results, respectively, from conventional culture-based drug susceptibility testing (DST). Sensitivity, specificity and overall accuracy of the FASTPlaque-Response test for rifampicin resistance were 98.1, 96.3 and 96.6 %, respectively, for all specimens with NOA, and 93.2, 96.3 and 95.9 % without NOA, when compared with resolved conventional DST results. Inclusion of the NOA supplement reduced contamination, increased the number of interpretable results and did not adversely affect the performance of the FASTPlaque-Response test. Thus, the use of NOA improves the robustness of the test, facilitating its wider implementation.

Improved contamination control for a rapid phage-based rifampicin resistance test for Mycobacterium tuberculosis. Publishing Authors By Initials

R Mole,A Trollip,C Abrahams,M Bosman,H Albert,R Mole,A Trollip,C Abrahams,M Bosman,H Albert,

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Improved contamination control for a rapid phage-based rifampicin resistance test for Mycobacterium tuberculosis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of medical microbiology

VOLUME: 56

Page Numbers: 1334-9

Journal Abbreviation: J. Med. Microbiol.

ISSN: 0022-2615

DAY: 25

MONTH: Oct

YEAR: 2007

Improved contamination control for a rapid phage-based rifampicin resistance test for Mycobacterium tuberculosis. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Improved contamination control for a rapid phage-based rifampicin resistance test for Mycobacterium tuberculosis.

AFFILIATION: Biotec Laboratories Ltd, Somerset Hospital, Greenpoint, Cape Town, South Africa.

Country: England

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MEDLINETA: J Med Microbiol

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