Impairment of sodium balance in mice deficient in renal principal cell mineralocorticoid receptor.

Impairment of sodium balance in mice deficient in renal principal cell mineralocorticoid receptor. Research Abstract Details 

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Impairment of sodium balance in mice deficient in renal principal cell mineralocorticoid receptor. Abstract Text:

Caroline Ronzaud,Johannes Loffing,Markus Bleich,Norbert Gretz,Hermann-Josef , ,Stefan Berger,Caroline Ronzaud,Johannes Loffing,Markus Bleich,Norbert Gretz,Hermann-Josef , ,Stefan Berger,

Germline inactivation of the mineralocorticoid receptor (MR) gene in mice results in postnatal lethality as a result of massive loss of sodium and water. The knockout mice show impaired epithelial sodium channel (ENaC) activity in kidney and colon. For determination of the role of renal MR in aldosterone-driven ENaC-mediated sodium reabsorption, mice with principal cell MR deficiency were generated using the Cre-loxP system. For driving Cre recombinase expression in principal cells, the regulatory elements of the mouse aquaporin 2 (AQP2) gene were used. Mutant mice (MR(AQP2Cre)) were obtained by crossing AQP2Cre mice with mice that carried a conditional MR allele. Under standard diet, MR(AQP2Cre) mice develop normally and exhibit unaltered renal sodium excretion but show strongly elevated aldosterone levels. Increased renal sodium and water excretion, resulting in continuous loss of body weight, occur under low-sodium diet. Immunofluorescence revealed that the loss of MR and apical ENaC staining is restricted to principal cells of the collecting duct (CD) and late connecting tubule (CNT) and that MR is crucial for ENaC trafficking to the apical membrane. These results demonstrate that inactivation of MR in CD and late CNT can be compensated under standard diet but no longer when sodium supply is limited. Because the mutant mice show preserved renal ENaC activity, this study provides evidence that the late distal convoluted tubule and early CNT can compensate to a large extent deficient ENaC-mediated sodium reabsorption in late CNT and CD.

Impairment of sodium balance in mice deficient in renal principal cell mineralocorticoid receptor. Publishing Authors By Initials

C Ronzaud,J Loffing,M Bleich,N Gretz,HJ ,G ,S Berger,C Ronzaud,J Loffing,M Bleich,N Gretz,HJ ,G ,S Berger,

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Impairment of sodium balance in mice deficient in renal principal cell mineralocorticoid receptor. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Society of Nephrology : JA

VOLUME: 18

Page Numbers: 1679-87

Journal Abbreviation: J. Am. Soc. Nephrol.

ISSN: 1046-6673

DAY: 2

MONTH: 05

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 9013836

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AFFILIATION: Division of Molecular Biology of the Cell I (A020), German Cancer Research Center, Im Neuenheimer Feld 581, 69120 Heidelberg, Germany.

Country: United States

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MEDLINETA: J Am Soc Nephrol

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