Impairment by activation of TRPA1 of gastric epithelial restitution in a wound model using RGM1 cell monolayer.

Impairment by activation of TRPA1 of gastric epithelial restitution in a wound model using RGM1 cell monolayer. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Impairment by activation of TRPA1 of gastric epithelial restitution in a wound model using RGM1 cell monolayer. Abstract Text:

S Hayashi,E Nakamura,T Endo,Y Kubo,K Takeuchi,S Hayashi,E Nakamura,T Endo,Y Kubo,K Takeuchi,

We examined the influence of TRPA1 on the epithelial restitution using a rat gastric epithelial cell line RGM1 monolayer. RGM1 cells were inoculated in 24-well plates cultured for 24 hr, and then starved for 24 hr in a culture medium at 37 degrees C under 5 % CO(2) in air. After obtaining a confluent RGM1 cell monolayer, a round artificial wound of constant size was induced in the center of the cell monolayer using a pencil-type mixer with a rotating silicon tip. The repair process was monitored by quantitatively measuring the area of the epithelial wound (cell-free area). Immediately after the wound induction, cells at the edge of wound started to form lamellipodia, migrating toward the center of wound, and by so doing the cell-free area was decreased over time. The addition of icilin, the TRPA1 agonist, suppressed the recovery of the epithelial wound in a concentration-dependent manner. Likewise, another TRPA1 agonist, ally isothiocyanate, also significantly inhibited the wound repair. In addition, the recovery of the epithelial wound was potently inhibited when the ambient temperature was lowered to 17 degrees C, the threshold temperature where TRPA1 is known to be activated. By contrast, the wound healing was not affected by either menthol, the TRPM8 agonist, or capsaicin, the TRPV1 agonist. These results showed for the first time that the activation of TRPA1 inhibited the repair of the epithelial wound in the stomach, probably by the suppression of cell migration, and suggested the involvement of TRPA1 in the mechanism of gastric epithelial restitution.

Impairment by activation of TRPA1 of gastric epithelial restitution in a wound model using RGM1 cell monolayer. Publishing Authors By Initials

S Hayashi,E Nakamura,T Endo,Y Kubo,K Takeuchi,S Hayashi,E Nakamura,T Endo,Y Kubo,K Takeuchi,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Impairment by activation of TRPA1 of gastric epithelial restitution in a wound model using RGM1 cell monolayer. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Inflammopharmacology

VOLUME: 15

Page Numbers: 218-22

Journal Abbreviation:

ISSN: 0925-4692

DAY: 18

MONTH: Oct

YEAR: 2007

Impairment by activation of TRPA1 of gastric epithelial restitution in a wound model using RGM1 cell monolayer. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9112626

Impairment by activation of TRPA1 of gastric epithelial restitution in a wound model using RGM1 cell monolayer. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Impairment by activation of TRPA1 of gastric epithelial restitution in a wound model using RGM1 cell monolayer. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Impairment by activation of TRPA1 of gastric epithelial restitution in a wound model using RGM1 cell monolayer.

AFFILIATION: Department of Pharmacology and Experimental Therapeutics, Kyoto Pharmaceutical University, Misasagi, Yamashina, Kyoto 607-8414, Japan.

Country: Switzerland

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Inflammopharmacology

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Impairment by activation of TRPA1 of gastric epithelial restitution in a wound model using RGM1 cell monolayer Related Publications

• A nationwide study of discrimination and chronic health conditions among Asian Americans.
• Allogenic mixed chimerism induced by nonlymphoablative regimen including donor BMT with low-dose TBI and anti-CD40L cured proliferative glomerulonephritis in lupus mice.
• Clustered negative charges on the lipid membrane surface induce beta-sheet formation of prion protein fragment 106-126.
• Cyclooxygenase-2/prostaglandin E2 accelerates the healing of gastric ulcers via EP4 receptors.
• Effect of dopamine on the healing of acetic acid-induced gastric ulcers in rats.
• Formation of giant lipid vesiclelike compartments from a planar lipid membrane by a pulsed jet flow.
• Health insurance coverage for vulnerable populations: contrasting Asian Americans and Latinos in the United States.
• Impairment by activation of TRPA1 of gastric epithelial restitution in a wound model using RGM1 cell monolayer.
• Increased susceptibility of small intestine to NSAID-provoked ulceration in rats with adjuvant-induced arthritis: involvement of enhanced expression of TLR4.
• Involvement of prostaglandin E receptor EP3 subtype and prostacyclin IP receptor in decreased acid response in damaged stomach.
• Involvement of prostaglandin E receptor EP3 subtype in duodenal bicarbonate secretion in rats.
• Mental health and substance use disorders among Latino and Asian American lesbian, gay, and bisexual adults.
• Molecularly imprinted polymer-assisted refolding of lysozyme.
• Pathogenic importance of pepsin in ischemia/reperfusion-induced gastric injury.
• Prevalence and correlates of eating disorders among Asian Americans: results from the National Latino and Asian American Study.
• Profiles of a pathogenic Entamoeba histolytica-like variant with variations in the nucleotide sequence of the small subunit ribosomal RNA isolated from a primate (De Brazza's guenon).
• Prophylactic effect of lafutidine against the adverse reaction induced in rat stomach by repeated administration of 5-fluorouracil.
• Prophylactic effect of rebamipide against the irritative and healing impairment actions of alendronate in rat stomachs.
• Prostaglandin E2 stimulates VEGF expression in primary rat gastric fibroblasts through EP4 receptors.
• Psychiatric disorders and labor market outcomes: evidence from the National Latino and Asian American Study.
• Relationships between self-reported unfair treatment and prescription medication use, illicit drug use, and alcohol dependence among Filipino Americans.
• Self-rated health among foreign- and U.S.-born Asian Americans: a test of comparability.
• Smoking prevalence among asian americans: findings from the National Latino and Asian American Study (NLAAS).
• Stimulation of Smad1 transcriptional activity by Ras-extracellular signal-regulated kinase pathway: a possible mechanism for collagen-dependent osteoblastic differentiation.
• Stimulatory effect of Coca-Cola on gastroduodenal HCO3- secretion in rats.
• Survey conditioning in self-reported mental health service use: randomized comparison of alternative instrument formats.
• Symptomatic Hyponatremia after Voluntary Excessive Water Ingestion in a Patient without Psychiatric Problems.
• The efficient use of microcolumns in chromatographic systems.
• [Pathophysiology in rickets/osteomalacia]
• [Ureteral avulsion: a rare complication of ureteroscopy]