Impact of salt bridges on the equilibrium binding and adhesion of human CD2 and CD58.

Impact of salt bridges on the equilibrium binding and adhesion of human CD2 and CD58. Research Abstract Details 

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Impact of salt bridges on the equilibrium binding and adhesion of human CD2 and CD58. Abstract Text:

Marco V Bayas,Alice Kearney,Adam Avramovic,P Anton van der Merwe,Deborah E Leckband,

This study describes quantitative investigations of the impact of single charge mutations on equilibrium binding, kinetics, and the adhesion strength of the CD2-CD58 interaction. Previously steered molecular dynamics simulations guided the selection of the charge mutants investigated, which include the CD2 mutants D31A, K41A, K51A, and K91A. This set includes mutations in which the previous cell aggregation and binding data either agreed or disagreed with the steered molecular dynamics predictions. Surface plasmon resonance measurements quantified the solution binding properties. Adhesion was quantified with the surface force apparatus, which was used previously to study the closely related CD2-CD48 interaction. The results reveal roles that these salt bridges play in equilibrium binding and adhesion. We discuss both the molecular basis of this behavior and its implications for cell adhesion.

Impact of salt bridges on the equilibrium binding and adhesion of human CD2 and CD58. Publishing Authors By Initials

MV Bayas,A Kearney,A Avramovic,PA van der Merwe,DE Leckband,

For similar inorganic chemicals: salts research abstracts see: inorganic chemicals: salts research

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Impact of salt bridges on the equilibrium binding and adhesion of human CD2 and CD58. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 5589-96

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 15

MONTH: 12

YEAR: 2006

Impact of salt bridges on the equilibrium binding and adhesion of human CD2 and CD58. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Impact of salt bridges on the equilibrium binding and adhesion of human CD2 and CD58. Keywords Mesh Terms:

KEYWORDS: Salts

MESH TERMS: metabolism

Chemical & Substance for Abstract: Impact of salt bridges on the equilibrium binding and adhesion of human CD2 and CD58. Information

Substance Name: Salts

Registry Number: 0

Grant and Affiliation Information for Impact of salt bridges on the equilibrium binding and adhesion of human CD2 and CD58.

AFFILIATION: Department of Chemistry and Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM51338

ACRONYM: GM

MEDLINETA: J Biol Chem

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