Impact of methodological factors on sperm penetration into cervical mucus in cattle.

Impact of methodological factors on sperm penetration into cervical mucus in cattle. Research Abstract Details 

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Impact of methodological factors on sperm penetration into cervical mucus in cattle. Abstract Text:

T Murase,J W Braun,

The aim of this study was to investigate the influence of methodological factors on the interaction of bovine spermatozoa and homologous cervical mucus. Cervical mucus was obtained from three cows during estrus. To evaluate the penetration ability of frozen-thawed semen samples of five different bulls, fresh mucus as well as frozen-thawed mucus, stored for 1, 10 or 30 d in liquid nitrogen, were used. Penetration assays were performed at 38 degrees C for 10 min, and the most advanced spermatozoon was located and the distance determined. Semen parameters were examined by a computer-assisted videomicrographic system. Conservation of mucus in liquid nitrogen for up to 30 d did not influence the results of the penetration assay. In contrast, the mucus of individual cows showed significant differences in the migration distance of spermatozoa. Sperm concentration, mean velocity and number of forward moving spermatozoa were significantly correlated with mucus penetration. These results demonstrated that the mucus penetration assay in cattle can be performed by dividing a mucus sample from a cow into many portions and storing the sample in liquid nitrogen.

Impact of methodological factors on sperm penetration into cervical mucus in cattle. Publishing Authors By Initials

T Murase,JW Braun,

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Impact of methodological factors on sperm penetration into cervical mucus in cattle. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 34

Page Numbers: 73-80

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Jul

YEAR: 1990

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LANGUAGE: eng

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AFFILIATION: Gynäkologische Tierklinik der Universität München Königinstr. 12, 8000 München 22, Federal Republic of Germany.

Country: United States

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MEDLINETA: Theriogenology

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