Impact of gamma irradiation on the transformation efficiency for extracellular plasmid DNA.

Impact of gamma irradiation on the transformation efficiency for extracellular plasmid DNA. Research Abstract Details 

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Impact of gamma irradiation on the transformation efficiency for extracellular plasmid DNA. Abstract Text:

Nobuyoshi Ishii,Testuya Sakashita,Hiroshi Takeda,Yoshihisa Kubota,Shoichi Fuma,Masahiro Doi,Sentaro Takahashi,Nobuyoshi Ishii,Testuya Sakashita,Hiroshi Takeda,Yoshihisa Kubota,Shoichi Fuma,Masahiro Doi,Sentaro Takahashi,

Extracellular DNA is omnipresent in aquatic environments and is thought to be a genetic material for horizontal gene transformation between microorganisms. We studied the impact of gamma irradiation on the transformation efficiency (transformants number per ng of DNA per ml) of extracellular DNA. Plasmid pEGFP as a model extracellular DNA was irradiated by gamma rays. The transformation efficiency decreased with the increase in radiation dose. A total dose of 10Gy is normally not lethal for microorganisms but certainly affects the transformation efficiency of extracellular DNA. The decrease in the efficiency would be induced by strand breaks of extracellular DNA because the yield of both single-strand breaks (SSBs) and double-strand breaks (DSBs) increased with the increase in radiation dose. The relative transformation efficiency of SSBs and DSBs to that of covalently closed circles (CCCs) was 30.3% and 0.2%, respectively. This impact on natural transformation suggests an inability of microorganisms to acquire new characteristics which should be normally acquired.

Impact of gamma irradiation on the transformation efficiency for extracellular plasmid DNA. Publishing Authors By Initials

N Ishii,T Sakashita,H Takeda,Y Kubota,S Fuma,M Doi,S Takahashi,N Ishii,T Sakashita,H Takeda,Y Kubota,S Fuma,M Doi,S Takahashi,

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Impact of gamma irradiation on the transformation efficiency for extracellular plasmid DNA. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of environmental radioactivity

VOLUME: 97

Page Numbers: 159-67

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ISSN: 0265-931X

DAY: 1

MONTH: 06

YEAR: 2007

Impact of gamma irradiation on the transformation efficiency for extracellular plasmid DNA. Information

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LANGUAGE: eng

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AFFILIATION: Environmental and Toxicological Sciences Research Group, National Institute of Radiological Sciences, Anagawa 4-9-1, Chiba 263-8555, Japan. nobu@nirs.go.jp

Country: England

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MEDLINETA: J Environ Radioact

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