Immunostaining on thin-layer chromatograms of oligosaccharides released from gangliosides by endoglycoceramidase.

Immunostaining on thin-layer chromatograms of oligosaccharides released from gangliosides by endoglycoceramidase. Research Abstract Details 

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Immunostaining on thin-layer chromatograms of oligosaccharides released from gangliosides by endoglycoceramidase. Abstract Text:

H Higashi,Y Hirabayashi,M Ito,T Yamagata,M Matsumoto,S Ueda,S Kato,

A method for immobilizing oligosaccharides on a TLC plate for immunostaining has been developed. N-Glycolylneuraminic acid (NeuGc)-containing oligosaccharides derived from II3NeuGc-LacCer, IV3NeuGc-nLcOse4Cer, II3NeuGc-GgOse3Cer, and II3(NeuGc)2-LacCer by digestion with our newly isolated endoglycoceramidase (Ito, M. & Yamagata, T. (1986) J. Biol. Chem. 261, 14278-14282) and sialyllactose were chromatographed on polyamide 11 TLC or NH2-HPTLC plates, and covalently linked to the plates by reductive amination with sodium cyanoborohydride (NaBH3-CN). The immobilized oligosaccharides were detected by enzyme-immunostaining using NeuGc-specific chicken anti-NeuGc-LacCer and horseradish peroxidase-conjugated rabbit anti-chicken IgG. II3NeuGc-nLcOse4 showed the highest reactivity with the antibody, followed by II3NeuGc-GgOse3. As little as 0.8 nmol of the NeuGc-containing oligosaccharides was detected. The polyamide 11 TLC aluminum plate was found to be more suitable for the immunostaining than the NH2-HPTLC plate under the conditions used. For binding of the oligosaccharides to the NH2-HPTLC plate, reductive amination was found to be superior to the heating method reported earlier.

Immunostaining on thin-layer chromatograms of oligosaccharides released from gangliosides by endoglycoceramidase. Publishing Authors By Initials

H Higashi,Y Hirabayashi,M Ito,T Yamagata,M Matsumoto,S Ueda,S Kato,

For similar animals: chordata: vertebrates: mammals: artiodactyla: swine research abstracts see: animals: chordata: vertebrates: mammals: artiodactyla: swine research

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Immunostaining on thin-layer chromatograms of oligosaccharides released from gangliosides by endoglycoceramidase. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 102

Page Numbers: 291-6

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1987

Immunostaining on thin-layer chromatograms of oligosaccharides released from gangliosides by endoglycoceramidase. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Immunostaining on thin-layer chromatograms of oligosaccharides released from gangliosides by endoglycoceramidase. Keywords Mesh Terms:

KEYWORDS: Swine

MESH TERMS: immunology

Chemical & Substance for Abstract: Immunostaining on thin-layer chromatograms of oligosaccharides released from gangliosides by endoglycoceramidase. Information

Substance Name: endoglycoceramidase

Registry Number: EC 3.2.1.123

Grant and Affiliation Information for Immunostaining on thin-layer chromatograms of oligosaccharides released from gangliosides by endoglycoceramidase.

AFFILIATION: Department of Pathology, Osaka University.

Country: JAPAN

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MEDLINETA: J Biochem

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