Immunolocalization of 5alpha-reductase isozymes in acne lesions and normal skin.

Immunolocalization of 5alpha-reductase isozymes in acne lesions and normal skin. Research Abstract Details 

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Immunolocalization of 5alpha-reductase isozymes in acne lesions and normal skin. Abstract Text:

BACKGROUND: Dihydrotestosterone mediates androgen-dependent diseases, such as acne, hirsutism, and androgenetic alopecia. This hormone is produced from testosterone by the 5alpha-reductase enzyme. There are 2 isozymes of 5alpha-reductase (types 1 and 2) that differ in their localization within the body and even within the skin. Activity of the type 1 isozyme predominates in sebaceous glands, where it may be involved in regulation of sebum production. Since specific inhibition of 5alpha-reductase type 1 may represent a novel therapeutic approach to acne, it is important to define the localization of these isozymes in normal sebaceous follicles and acne lesions. OBSERVATIONS: Skin biopsy specimens were obtained from the backs of 11 subjects: 8 with acne and 3 without acne. Sections of normal follicles, open comedones, closed comedones, and inflammatory lesions were incubated with antibodies to types 1 and 2 5alpha-reductase. In all samples, the type 1 antibody localized specifically to sebaceous glands, and the type 2 antibody localized to the companion layer of the hair follicle (the innermost layer of the outer root sheath) and granular layer of the epidermis. Localization of the type 2 isozyme was also noted within the walls of open and closed comedones and in endothelial cells from sections of inflammatory lesions. CONCLUSIONS: The immunolocalization of 5alpha-reductase isozymes in normal sebaceous follicles and acne follicles is similar to the pattern described in terminal hair follicles and corresponds with the findings of biochemical studies that have demonstrated predominance of type 1 activity in sebaceous glands. The function of type 2 5alpha-reductase in comedones or endothelial cells in inflammatory lesions is unknown.

Immunolocalization of 5alpha-reductase isozymes in acne lesions and normal skin. Publishing Authors By Initials

For similar enzymes and coenzymes: enzymes: oxidoreductases: oxidoreductases acting on ch-ch group donors: testosterone 5-alpha-reductase research abstracts see: enzymes and coenzymes: enzymes: oxidoreductases: oxidoreductases acting on ch-ch group donors: testosterone 5-alpha-reductase research

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Immunolocalization of 5alpha-reductase isozymes in acne lesions and normal skin. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Archives of dermatology

VOLUME: 136

Page Numbers: 1125-9

Journal Abbreviation: Arch Dermatol

ISSN: 0003-987X

DAY: 17

MONTH: Sep

YEAR: 2000

Immunolocalization of 5alpha-reductase isozymes in acne lesions and normal skin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 372433

Immunolocalization of 5alpha-reductase isozymes in acne lesions and normal skin. Keywords Mesh Terms:

KEYWORDS: Testosterone 5-alpha-Reductase

MESH TERMS: analysis

Chemical & Substance for Abstract: Immunolocalization of 5alpha-reductase isozymes in acne lesions and normal skin. Information

Substance Name: Testosterone 5-alpha-Reductase

Registry Number: EC 1.3.99.5

Grant and Affiliation Information for Immunolocalization of 5alpha-reductase isozymes in acne lesions and normal skin.

AFFILIATION: Section of Dermatology, UPC-2, Room 4300, Pennsylvania State University College of Medicine, 500 University Dr, Hershey, PA 17033, USA. dthiboutot@psu.edu

Country: UNITED STATES

AGENCY: United States NIAMS

GRANT: 1-K08-AR-02018

ACRONYM: AR

MEDLINETA: Arch Dermatol

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