Immunohistochemical demonstration of advanced glycation end products and the effects of advanced glycation end products in ossified ligament tissues in vitro.

Immunohistochemical demonstration of advanced glycation end products and the effects of advanced glycation end products in ossified ligament tissues in vitro. Research Abstract Details 

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Immunohistochemical demonstration of advanced glycation end products and the effects of advanced glycation end products in ossified ligament tissues in vitro. Abstract Text:

Kimiaki Yokosuka,Jin Soo Park,Kotaro Jimbo,Tatuhiro Yoshida,Kei Yamada,Kimiaki Sato,Masayoshi Takeuchi,Sho-ichi Yamagishi,Kensei Nagata,Kimiaki Yokosuka,Jin Soo Park,Kotaro Jimbo,Tatuhiro Yoshida,Kei Yamada,Kimiaki Sato,Masayoshi Takeuchi,Sho-ichi Yamagishi,Kensei Nagata,

STUDY DESIGN: This study correlates advanced glycation end products with ossified ligament tissues of the cervical spine in vitro. OBJECTIVE: To investigate the effect of advanced glycation end products on ossification of the spinal ligaments in vitro. SUMMARY OF BACKGROUND DATA: We have hypothesized that an accumulation of advanced glycation end products in the spinal ligament might result in some observable change in specific growth factors responsible for ossification in the spinal ligaments. METHODS: Samples of the posterior longitudinal and yellow ligaments were harvested from patients (n = 5) with ossification of the posterior longitudinal ligament, and analyzed for the presence of advanced glycation end products and their receptor advanced glycation end product receptor by immunohistochemistry. Real-time polymerase chain reaction (PCR) was used to quantify the messenger ribonucleic acid (mRNA) levels of bone morphogenetic protein (BMP)-2, BMP-7, alkaline phosphatase, an osteoblast-specific transcription factor 1 (Cbfa1), and osteocalcin from yellow ligament cells treated with advanced glycation end products. RESULTS: Immunohistochemical analysis revealed that advanced glycation end products and advanced glycation end product receptor were localized to within the posterior longitudinal and yellow ligaments. Advanced glycation end products were found to increase significantly the expression of BMP-2, BMP-7, Cbfa1, and osteocalcin at the mRNA levels after treatment with advanced glycation end products (1 microg/mL). CONCLUSIONS: This is the first report to investigate the correlation, if any, between the ossified spinal ligament and advanced glycation end products. These results suggested that accumulation in advanced glycation end products and their interaction with advanced glycation end product receptor were 1 of the important risk factors in the process of ossification in the spinal ligaments.

Immunohistochemical demonstration of advanced glycation end products and the effects of advanced glycation end products in ossified ligament tissues in vitro. Publishing Authors By Initials

K Yokosuka,JS Park,K Jimbo,T Yoshida,K Yamada,K Sato,M Takeuchi,S Yamagishi,K Nagata,K Yokosuka,JS Park,K Jimbo,T Yoshida,K Yamada,K Sato,M Takeuchi,S Yamagishi,K Nagata,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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Immunohistochemical demonstration of advanced glycation end products and the effects of advanced glycation end products in ossified ligament tissues in vitro. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Spine

VOLUME: 32

Page Numbers: E337-9

Journal Abbreviation: Spine

ISSN: 1528-1159

DAY: 15

MONTH: May

YEAR: 2007

Immunohistochemical demonstration of advanced glycation end products and the effects of advanced glycation end products in ossified ligament tissues in vitro. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7610646

Immunohistochemical demonstration of advanced glycation end products and the effects of advanced glycation end products in ossified ligament tissues in vitro. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: metabolism

Chemical & Substance for Abstract: Immunohistochemical demonstration of advanced glycation end products and the effects of advanced glycation end products in ossified ligament tissues in vitro. Information

Substance Name: Alkaline Phosphatase

Registry Number: EC 3.1.3.1

Grant and Affiliation Information for Immunohistochemical demonstration of advanced glycation end products and the effects of advanced glycation end products in ossified ligament tissues in vitro.

AFFILIATION: Department of Orthopedic Surgery, Kurume University School of Medicine, Kurume, Japan.

Country: United States

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MEDLINETA: Spine

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