Immunohistochemical and in situ hybridization observations favor a local catecholamine production in the human Achilles tendon.

Immunohistochemical and in situ hybridization observations favor a local catecholamine production in the human Achilles tendon. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Immunohistochemical and in situ hybridization observations favor a local catecholamine production in the human Achilles tendon. Abstract Text:

Dennis Bjur,Patrik Danielson, Alfredson,Sture Forsgren,Dennis Bjur,Patrik Danielson, Alfredson,Sture Forsgren,

Results of recent studies using immunohistochemistry show evidence of an occurrence of catecholamine production in the cells (tenocytes) of patellar tendons exhibiting tendinopathy (tendinosis). In the present study, antibodies against the catecholamine-synthesizing enzyme tyrosine hydroxylase (TH) and alpha1-adrenoreceptors were applied to sections of specimens of normal and tendinosis Achilles tendons. In situ hybridization using a probe detecting human TH mRNA was also utilized. It was found that sympathetic innervation was very scarce. On the other hand, there were distinct alpha1-adrenoreceptor immunoreactions in blood vessel walls. Interestingly, tenocytes, particularly from tendinosis samples in which the tenocytes showed an abnormal shape (not the typical slender appearance), displayed TH immunoreactions and reactions for TH mRNA. Of further interest was the finding of alpha1-adrenoreceptor immunoreactions in tenocytes. The observations show not only evidence of local catecholamine production at the protein level, which was the case in recent studies for the patellar tendon, but also at the mRNA level. The observations suggest that the tenocytes, especially those with disfigured appearances in tendinosis, can produce catecholamines and also that they can respond to sympathetic transmitters. This is of interest as adrenergic stimulation in other parts of the body is known to induce degenerative/apoptotic and proliferative events, features which are seen in Achilles tendinosis. These observations are completely new findings concerning the human Achilles tendon. It is likely that locally produced catecholamines and the occurrence of autocrine/paracrine effects of these substances are of great relevance during the process of tendinosis.

Immunohistochemical and in situ hybridization observations favor a local catecholamine production in the human Achilles tendon. Publishing Authors By Initials

D Bjur,P Danielson,H Alfredson,S Forsgren,D Bjur,P Danielson,H Alfredson,S Forsgren,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Immunohistochemical and in situ hybridization observations favor a local catecholamine production in the human Achilles tendon. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Histology and histopathology

VOLUME: 23

Page Numbers: 197-208

Journal Abbreviation:

ISSN: 1699-5848

DAY: 13

MONTH: Feb

YEAR: 2008

Immunohistochemical and in situ hybridization observations favor a local catecholamine production in the human Achilles tendon. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8609357

Immunohistochemical and in situ hybridization observations favor a local catecholamine production in the human Achilles tendon. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Immunohistochemical and in situ hybridization observations favor a local catecholamine production in the human Achilles tendon. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Immunohistochemical and in situ hybridization observations favor a local catecholamine production in the human Achilles tendon.

AFFILIATION: Department of Integrative Medical Biology, Section for Anatomy, Umeå University, Umeå, Sweden.

Country: Spain

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Histol Histopathol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Immunohistochemical and in situ hybridization observations favor a local catecholamine production in the human Achilles tendon Related Publications

• A skin chamber technique for leukocyte migration studies; description and reproducibility.
• Accuracy of computed tomography in the diagnosis of herpes simplex encephalitis.
• Alpha-azidobenzylpenicillin. I. Chemistry, bacteriology, and experimental chemotherapy.
• Are neuropeptides important in arthritis? Studies on the importance of bombesin/GRP and substance P in a murine arthritis model.
• BDNF in RA: Downregulated in plasma following anti-TNF treatment but no correlation with inflammatory parameters.
• Conservative treatment of splenic injuries.
• Enzyme-linked immunosorbent assay (ELISA) for antibodies against Campylobacter jejuni, and its clinical application.
• Estimations of the prevalence of epilepsy in sub-Saharan Africa.
• Functional properties of polymorphonuclear leukocytes accumulated in a skin chamber.
• Further in vitro studies of azidocillin against Haemophilus.
• Hormonal regulation of female nuptial coloration in a fish.
• Immunohistochemical and in situ hybridization observations favor a local catecholamine production in the human Achilles tendon.
• In situ hybridization studies confirming recent findings of the existence of a local nonneuronal catecholamine production in human patellar tendinosis.
• Labelled tocopherols in man. Intestinal absorption and thoracic-duct lymph transport of dl-alpha-tocopheryl-3,4-14C2 acetate dl-alpha-tocopheramine-3,4-14C2 dl-alpha-tocopherol-(5-methyl-3H) and N-(methyl-3H)-dl-gamma-tocopheramine.
• Marked sympathetic component in the perivascular innervation of the dorsal paratendinous tissue of the patellar tendon in arthroscopically treated tendinosis patients.
• Nerve-related characteristics of ventral paratendinous tissue in chronic Achilles tendinosis.
• New aspects concerning ulcerative colitis and colonic carcinoma: Analysis of levels of neuropeptides, neurotrophins, and TNFalpha/TNFreceptor in plasma and mucosa in parallel with histological evaluation of the intestine.
• Observations favouring the occurrence of local production and marked effects of bombesin/gastrin-releasing peptide in the synovial tissue of the human knee joint - Comparisons with substance P and the NK-1 receptor.
• Passage of benzylpenicillin and ampicillin into the thoracic duct lymph after intravenous injection in man.
• Presence of a marked nonneuronal cholinergic system in human colon: study of normal colon and colon in ulcerative colitis.
• Presence of a non-neuronal cholinergic system and occurrence of up- and down-regulation in expression of M2 muscarinic acetylcholine receptors: new aspects of importance regarding Achilles tendon tendinosis (tendinopathy).
• Presence of substance P and the neurokinin-1 receptor in tenocytes of the human Achilles tendon.
• Renal plasma flow during moderate exercise of several hours' duration in normal male subjects.
• Studies on the intestinal absorption of labelled fat-soluble vitamins (A, D, E, and K) via the thoracic-duct lymph in the absence of bile in man.
• Unexpected finding of a marked non-neuronal cholinergic system in human knee joint synovial tissue.
• VGluT2 expression in painful Achilles and patellar tendinosis: Evidence of local glutamate release by tenocytes.
• Vitamin K1-3H in man. Its intestinal absorption and transport in the thoracic duct lymph.
• [Maternal, congenital and neonatal acquired cytomegalic virus infections]
• [Not Available.]
• [Word processing of medical records and epicrises at a hospital department]