Immunogenicity of porcine circovirus type 2 capsid protein targeting to different subcellular compartments.

Immunogenicity of porcine circovirus type 2 capsid protein targeting to different subcellular compartments. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Immunogenicity of porcine circovirus type 2 capsid protein targeting to different subcellular compartments. Abstract Text:

Huiying Fan,Shaobo Xiao,Tiezhu Tong,Shengpin Wang,Lilan Xie,Yunbo Jiang,Huanchun Chen,Liurong Fang,Huiying Fan,Shaobo Xiao,Tiezhu Tong,Shengpin Wang,Lilan Xie,Yunbo Jiang,Huanchun Chen,Liurong Fang,Huiying Fan,Shaobo Xiao,Tiezhu Tong,Shengpin Wang,Lilan Xie,Yunbo Jiang,Huanchun Chen,Liurong Fang,Huiying Fan,Shaobo Xiao,Tiezhu Tong,Shengpin Wang,Lilan Xie,Yunbo Jiang,Huanchun Chen,Liurong Fang,

Porcine circovirus type 2 (PCV2) is known to be associated with post-weaning multisystemic wasting syndrome (PMWS), an emerging disease in swine. The development of effective vaccines against PCV2 infection has been accepted as an important strategy in the prophylaxis of PMWS, and a DNA vaccine expressing the major immunogenic capsid (Cap) protein of PCV2 is considered to be a promising candidate. However, recent studies have revealed that interferons (IFNs), especially IFN-gamma, can enhance the replication of PCV2, indicating that the high levels of IFN-gamma induced by DNA vaccination seem to have potential deleterious effect on protective immunity. Strategies to improve the neutralizing antibody response and simultaneously decrease the IFN-gamma response will facilitate the clinical application of DNA vaccines against PCV2. In the present study, four different DNA vaccine constructs encoding cytoplasmic (Cy-ORF2), secreted (Sc-ORF2), membrane-anchored (M-ORF2) or authentic nuclear-targeted (pc-ORF2) Cap protein were generated to evaluate the neutralizing antibody and IFN-gamma responses in a mouse model. Although all four DNA constructs could elicit PCV2-specific humoral immune responses, mice inoculated with Sc-ORF2 developed a significantly higher level of neutralizing antibodies than those that received M-ORF2, pc-ORF2 or Cy-ORF2. Furthermore, mice immunized with Sc-ORF2 or M-ORF2 showed a significantly decreased or enhanced IFN-gamma level, respectively, compared with those inoculated with pc-ORF2. With respect to neutralizing antibody and IFN-gamma levels, Sc-ORF2 is a good candidate for DNA vaccination, and the secreted Cap protein appears to be an ideal antigen for use in development of vaccines against PCV2.

Immunogenicity of porcine circovirus type 2 capsid protein targeting to different subcellular compartments. Publishing Authors By Initials

H Fan,S Xiao,T Tong,S Wang,L Xie,Y Jiang,H Chen,L Fang,H Fan,S Xiao,T Tong,S Wang,L Xie,Y Jiang,H Chen,L Fang,H Fan,S Xiao,T Tong,S Wang,L Xie,Y Jiang,H Chen,L Fang,H Fan,S Xiao,T Tong,S Wang,L Xie,Y Jiang,H Chen,L Fang,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Immunogenicity of porcine circovirus type 2 capsid protein targeting to different subcellular compartments. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Molecular immunology

VOLUME: 45

Page Numbers: 653-60

Journal Abbreviation: Mol. Immunol.

ISSN: 0161-5890

DAY: 20

MONTH: 08

YEAR: 2007

Immunogenicity of porcine circovirus type 2 capsid protein targeting to different subcellular compartments. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7905289

Immunogenicity of porcine circovirus type 2 capsid protein targeting to different subcellular compartments. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Immunogenicity of porcine circovirus type 2 capsid protein targeting to different subcellular compartments. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Immunogenicity of porcine circovirus type 2 capsid protein targeting to different subcellular compartments.

AFFILIATION: Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China; State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Mol Immunol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Immunogenicity of porcine circovirus type 2 capsid protein targeting to different subcellular compartments Related Publications

• Alterations of MUC1 and MUC3 expression in gastric carcinoma: relevance to patient clinicopathological features.
• Population structure and gene flow among wild populations of the black muntjac (Muntiacus crinifrons) based on mitochondrial DNA control region sequences.
• Transcriptional mRNA of bone morphogenetic proteins 2, 3, 4, and 5 in trigeminal nerve, benign and malignant peripheral nerve sheath tumors.
• [A clinical analysis of hepatolenticular degeneration in 42 patients]
• [A clinical study on delayed encephalopathy after carbon monoxide poisoning]
• [Analysis of C5 fractions and carbonyl compounds by capillary gas chromatography]
• [Clinical application of peroneal muscles tendon transposition in repair of Achilles tendon rupture]
• [Clinical significance of early diagnose and treatment of subclinical renal allograft rejection]
• [Clinical study on spinal cord injury without radiologic abnormality in children]
• [Comparison of various kinds of internal fixations in unstable intertrochanteric femoral fracture]
• [Determination of gamma-aminobutyric acid and glutamic acid in human cerebrospinal fluid by high performance liquid chromatography]
• [Distribution of neuropeptide Y-like and beta-endorphin-like immunoreactivity in the nervous system and Hatschek's pit of amphioxus, Branchiostoma belcheri]
• [Effect of Twin-block appliance in the treatment of Class II and division I malocclusion: a cephalometric study in 12 patients]
• [Investigation of an outbreak of acute diarrhea caused by human calicivirus]
• [Mechanism of oral absorption of panaxnotoginseng saponins]
• [Nine cases of laparoscopic retroperitoneal lymph node dissection]
• [Progress in application of surface enhanced Raman scattering spectrum technique]
• [Relationship between anatomy of knee colateral ligament and geometry of posterior femoral condylar articular surface]
• [Research on continuous measurement of blood pressure via characteristic parameters of pulse wave]
• [Studies on polysaccharide peptide with high performance gel permeation chromatography]
• [Studies on sequential injection spectrophotometric system using a reflective flow cell for process analysis]
• [Study of the photoluminescence spectra of laser dye impregnated in porous silicon]
• [Study on interaction of adriamycin and DNA with a resonance light scattering technique and its DNA analytical application]
• [Study on the NIR-Raman spectra and NIR-SERS spectra of amsacrine]
• [Systemic lupus erythematosus with Evan's syndrome as primary symptom in a case]
• [Temperature distribution measurement of high energetic monopropellant by spectroscopic diagnostic technology]
• [The changes in serum antibody level after immunization with HFRS vaccine]
• [The design of the external defibrillator using the truncated exponential biphasic waveform]
• [The historical condition in the spread of Sanqi (Panax notoginseng) in the Ming Dynasty]
• [The impact of expansion of sirolimus eluting stent on intimal hyperplasia: an intravascular ultrasound study]