Immunogenicity and antigenicity of conserved peptides from the envelope of HIV-1 expressed at the surface of recombinant bacteria.

Immunogenicity and antigenicity of conserved peptides from the envelope of HIV-1 expressed at the surface of recombinant bacteria. Research Abstract Details 

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Immunogenicity and antigenicity of conserved peptides from the envelope of HIV-1 expressed at the surface of recombinant bacteria. Abstract Text:

A Charbit,A Molla,J Ronco,J M ,V Favier,E M Bahraoui,L Montagnier,A Leguern,M Hofnung,

We expressed peptides from the HIV-1 envelope protein at the surface of Escherichia coli by genetic insertions into an exposed loop of the outer membrane protein LamB. Recombinant bacteria expressing eight peptides from gp110 (pep1-pep8), conserved between HIV-1 and HIV-2, were used as live immunogens in rabbits by the intravenous route. The eight constructions elicited anti-LamB antibodies, showing that the hybrid proteins were immunogenic. One of them, LamB-pep8, gave rise to antibodies able to react with gp160 and to neutralize HIV-1 in vitro. We also show that this type of recombinant E. coli can provide a convenient reagent to monitor and characterize specific antibodies. Recombinant clones were used to test sera of seropositive individuals, as well as to narrow down the monoclonal antibody 110-1 recognition site to a cluster of eight residues at the carboxy-terminal end of gp110.

Immunogenicity and antigenicity of conserved peptides from the envelope of HIV-1 expressed at the surface of recombinant bacteria. Publishing Authors By Initials

A Charbit,A Molla,J Ronco,JM ,V Favier,EM Bahraoui,L Montagnier,A Leguern,M Hofnung,

For similar proteins: viral proteins: viral structural proteins: viral envelope proteins research abstracts see: proteins: viral proteins: viral structural proteins: viral envelope proteins research

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Immunogenicity and antigenicity of conserved peptides from the envelope of HIV-1 expressed at the surface of recombinant bacteria. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: AIDS (London, England)

VOLUME: 4

Page Numbers: 545-51

Journal Abbreviation: AIDS

ISSN: 0269-9370

DAY: 27

MONTH: Jun

YEAR: 1990

Immunogenicity and antigenicity of conserved peptides from the envelope of HIV-1 expressed at the surface of recombinant bacteria. Information

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LANGUAGE: eng

NlmUniqueID: 8710219

Immunogenicity and antigenicity of conserved peptides from the envelope of HIV-1 expressed at the surface of recombinant bacteria. Keywords Mesh Terms:

KEYWORDS: Viral Envelope Proteins

MESH TERMS: immunology

Chemical & Substance for Abstract: Immunogenicity and antigenicity of conserved peptides from the envelope of HIV-1 expressed at the surface of recombinant bacteria. Information

Substance Name: maltoporins

Registry Number: 0

Grant and Affiliation Information for Immunogenicity and antigenicity of conserved peptides from the envelope of HIV-1 expressed at the surface of recombinant bacteria.

AFFILIATION: Unité de Programmation Moléculaire et Toxicologie Génétique (CNRS UA271, INSERM U163), Paris, France.

Country: UNITED STATES

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MEDLINETA: AIDS

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