Immunofluorescence localization of a 23-kDa Tetrahymena calcium-binding protein, TCBP-23, in the cell cortex.

Immunofluorescence localization of a 23-kDa Tetrahymena calcium-binding protein, TCBP-23, in the cell cortex. Research Abstract Details 

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Immunofluorescence localization of a 23-kDa Tetrahymena calcium-binding protein, TCBP-23, in the cell cortex. Abstract Text:

K Hanyu,O Numata,M Takahashi,Y Watanabe,

Previously, we succeeded in cloning a cDNA of the 23-kDa Tetrahymena Ca(2+)-binding protein, designated TCBP-23. Analysis of the deduced amino acid sequences showed that TCBP-23 is a member of the EF-hand family of Ca(2+)-binding proteins. However, its physiological function was not elucidated. In the studies reported here, recombinant TCBP-23 was expressed in Escherichia coli and purified. Since recombinant TCBP-23 binds Ca2+ in vitro, Ca(2+)-binding domains of the protein are likely to be functional in vivo. Rabbit antibodies against TCBP-23 were raised and used to determine the intracellular localization of the protein in Tetrahymena cells by indirect immunofluorescence. The antibodies strongly stained the whole cell cortex except for the oral apparatus and around the basal bodies. TCBP-23 remained in detergent-extracted cells, suggesting that it is associated with the epiplasm, the membrane skeleton of Tetrahymena. These results suggest that TCBP-23 may mediate Ca(2+)-regulated processes in the cell cortex.

Immunofluorescence localization of a 23-kDa Tetrahymena calcium-binding protein, TCBP-23, in the cell cortex. Publishing Authors By Initials

K Hanyu,O Numata,M Takahashi,Y Watanabe,

For similar animals: invertebrates: protozoa: ciliophora: oligohymenophorea: hymenostomatida: tetrahymenina: tetrahymena research abstracts see: animals: invertebrates: protozoa: ciliophora: oligohymenophorea: hymenostomatida: tetrahymenina: tetrahymena research

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Immunofluorescence localization of a 23-kDa Tetrahymena calcium-binding protein, TCBP-23, in the cell cortex. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 119

Page Numbers: 914-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1996

Immunofluorescence localization of a 23-kDa Tetrahymena calcium-binding protein, TCBP-23, in the cell cortex. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Immunofluorescence localization of a 23-kDa Tetrahymena calcium-binding protein, TCBP-23, in the cell cortex. Keywords Mesh Terms:

KEYWORDS: Tetrahymena

MESH TERMS: ultrastructure

Chemical & Substance for Abstract: Immunofluorescence localization of a 23-kDa Tetrahymena calcium-binding protein, TCBP-23, in the cell cortex. Information

Substance Name: Calcium

Registry Number: 7440-70-2

Grant and Affiliation Information for Immunofluorescence localization of a 23-kDa Tetrahymena calcium-binding protein, TCBP-23, in the cell cortex.

AFFILIATION: Institute of Biological Sciences, University of Tsukuba, Ibaraki.

Country: JAPAN

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MEDLINETA: J Biochem

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