Immunodominance of a recombinant T-cell epitope depends on its molecular environment.

Immunodominance of a recombinant T-cell epitope depends on its molecular environment. Research Abstract Details 

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Immunodominance of a recombinant T-cell epitope depends on its molecular environment. Abstract Text:

C Leclerc,P Martineau,A Charbit,R Lo-Man,E ,M Hofnung,

In the present study, we have investigated the influence of the molecular environment of a T-cell epitope on its immunogenicity. We genetically inserted into different sites of two bacterial recipient proteins, LamB or MalE, an immunodominant T-cell epitope: the 120-132 T-cell epitope from the PreS2 region of HBV. The T-cell epitope was introduced, either alone (PreS:T) or with an adjacent B-cell epitope (PreS:TB). After purification, the hybrid proteins were injected into mice and we studied the immunogenicity of recombinant T-cell epitopes by analyzing the in vitro proliferative responses of LN cells from these mice to the inserted peptides. The immunization of mice with recombinant MalE protein containing the PreS:T or PreS:TB peptides at two different sites induced strong peptide-specific proliferative responses, indicating that the insertion sites did not affect the immunodominance of the inserted T-cell epitope. A strong T-cell proliferative response was also obtained after immunization of mice with hybrid LamB protein containing the PreS:TB epitope at position 153. In contrast, the recombinant proteins which contained only the PreS:T epitope at positions 153 or 374 failed to stimulate T-cell responses. Therefore, this study demonstrates that the immunogenicity of recombinant T-cell epitopes may be strongly affected both by the insertion site and by inserted adjacent residues.

Immunodominance of a recombinant T-cell epitope depends on its molecular environment. Publishing Authors By Initials

C Leclerc,P Martineau,A Charbit,R Lo-Man,E ,M Hofnung,

For similar cells: blood cells: leukocytes: leukocytes, mononuclear: lymphocytes: t-lymphocytes research abstracts see: cells: blood cells: leukocytes: leukocytes, mononuclear: lymphocytes: t-lymphocytes research

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Immunodominance of a recombinant T-cell epitope depends on its molecular environment. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular immunology

VOLUME: 30

Page Numbers: 1561-72

Journal Abbreviation: Mol. Immunol.

ISSN: 0161-5890

DAY: 27

MONTH: Dec

YEAR: 1993

Immunodominance of a recombinant T-cell epitope depends on its molecular environment. Information

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LANGUAGE: eng

NlmUniqueID: 7905289

Immunodominance of a recombinant T-cell epitope depends on its molecular environment. Keywords Mesh Terms:

KEYWORDS: T-Lymphocytes

MESH TERMS: immunology

Chemical & Substance for Abstract: Immunodominance of a recombinant T-cell epitope depends on its molecular environment. Information

Substance Name: presurface protein 2, hepatitis B surfac

Registry Number: 0

Grant and Affiliation Information for Immunodominance of a recombinant T-cell epitope depends on its molecular environment.

AFFILIATION: Unité de Biologie des Régulations Immunitaires, Institut Pasteur, Paris, France.

Country: ENGLAND

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MEDLINETA: Mol Immunol

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