Immunocytochemical studies on the distribution pattern of daunomycin in rat gastrointestinal tract.

Immunocytochemical studies on the distribution pattern of daunomycin in rat gastrointestinal tract. Research Abstract Details 

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Immunocytochemical studies on the distribution pattern of daunomycin in rat gastrointestinal tract. Abstract Text:

Koji Ohara,Masashi Shin,Hiromichi Nakamuta,Lars-Inge Larsson,David M Hougaard,Kunio Fujiwara,Koji Ohara,Masashi Shin,Hiromichi Nakamuta,Lars-Inge Larsson,David M Hougaard,Kunio Fujiwara,Koji Ohara,Masashi Shin,Hiromichi Nakamuta,Lars-Inge Larsson,David M Hougaard,Kunio Fujiwara,

The cancer drug daunomycin is used in treatment of leukemia but possesses severe side effects that involve the gastrointestinal tract. We therefore used a newly developed immunocytochemical procedure to determine the distribution of DM in the gastrointestinal tracts of rats after i.v. injection. Two hours after injection, DM was diffusely distributed in nuclei and most parts of the cytoplasm of intestinal epithelial cells. The cytoplasmic immunoreactivity for DM was most pronounced in small granules of the apical cytoplasm. Sixteen hours after injection, DM immunostaining was by and large absent in the villous epithelium but persisted in the intestinal crypts. In addition, staining was also detected in endothelial cells, scattered cells of the lamina propria and in smooth muscle cells. After 5 days, only little staining for DM remained. Similar findings were made in the colon. In the gastric mucosa, DM accumulation persisted at 16 h in some glandular cells but was lost from the surface epithelium. No staining was detected in saline-injected control rats. The distribution of DM accumulation correlated partially with the distribution of apoptotic cells as detected by the TUNEL procedure. Our results pinpoint that DM may exert prolonged effects on glandular and regenerative cells of the gastrointestinal tract-an observation that may explain the gastrointestinal toxicity of the drug. It seems possible that DM accumulation in surface epithelial cells is rapidly cleared through drug transporters.

Immunocytochemical studies on the distribution pattern of daunomycin in rat gastrointestinal tract. Publishing Authors By Initials

K Ohara,M Shin,H Nakamuta,LI Larsson,DM Hougaard,K Fujiwara,K Ohara,M Shin,H Nakamuta,LI Larsson,DM Hougaard,K Fujiwara,K Ohara,M Shin,H Nakamuta,LI Larsson,DM Hougaard,K Fujiwara,

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Immunocytochemical studies on the distribution pattern of daunomycin in rat gastrointestinal tract. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Histochemistry and cell biology

VOLUME: 128

Page Numbers: 285-90

Journal Abbreviation: Histochem. Cell Biol.

ISSN: 0948-6143

DAY: 28

MONTH: 07

YEAR: 2007

Immunocytochemical studies on the distribution pattern of daunomycin in rat gastrointestinal tract. Information

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LANGUAGE: eng

NlmUniqueID: 9506663

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Grant and Affiliation Information for Immunocytochemical studies on the distribution pattern of daunomycin in rat gastrointestinal tract.

AFFILIATION: Department of Applied Life Science, Faculty of Biotechnology and Life Science, Sojo University, Ikeda 4-22-1, Kumamoto, 860-0082, Japan, fujiwara@life.sojo-u.ac.jp.

Country: Germany

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MEDLINETA: Histochem Cell Biol

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