Immunochemical evidence for the catalysis of vitamin D3 25-hydroxylation and testosterone 16 alpha-hydroxylation by homologous forms of cytochrome P-450 in rat liver microsomes.

Immunochemical evidence for the catalysis of vitamin D3 25-hydroxylation and testosterone 16 alpha-hydroxylation by homologous forms of cytochrome P-450 in rat liver microsomes. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Immunochemical evidence for the catalysis of vitamin D3 25-hydroxylation and testosterone 16 alpha-hydroxylation by homologous forms of cytochrome P-450 in rat liver microsomes. Abstract Text:

S Hayashi,T Omura,T Watanabe,K Okuda,

Polyclonal antibody elicited in a rabbit against purified cytochrome P-450cc25, which catalyzes 25-hydroxylation of vitamin D3, inhibited not only 25-hydroxylation of cholecalciferol and 1 alpha-hydroxycholecalciferol, but also 16 alpha- and 2 alpha-hydroxylation of testosterone catalyzed by the purified P-450cc25 preparation. Antibody inhibition experiments with microsomes revealed that most 16 alpha- and 2 alpha-hydroxylation of testosterone and most 25-hydroxylation of cholecalciferol by male rat liver microsomes were catalyzed by P-450cc25. In order to examine the identity of cholecalciferol 25-hydroxylase and testosterone 16 alpha-hydroxylase, monoclonal antibodies recognizing three different epitopes of P-450cc25 were prepared from hybridoma clones produced by fusion of mouse myeloma cells (P3X63Ag8U1) with the spleen cells of immunized BALB/c mouse. All of these monoclonal antibodies inhibited both 25-hydroxylation of 1 alpha-hydroxycholecalciferol and 16 alpha-hydroxylation of testosterone by purified P-450cc25. These observations suggested that immunochemically indistinguishable form(s) of cytochrome P-450 catalyzed both reactions.

Immunochemical evidence for the catalysis of vitamin D3 25-hydroxylation and testosterone 16 alpha-hydroxylation by homologous forms of cytochrome P-450 in rat liver microsomes. Publishing Authors By Initials

S Hayashi,T Omura,T Watanabe,K Okuda,

For similar enzymes and coenzymes: cytochromes: cytochrome p-450 enzyme system: steroid hydroxylases research abstracts see: enzymes and coenzymes: cytochromes: cytochrome p-450 enzyme system: steroid hydroxylases research

PUBMED ID PMID:

MEDLINE DATE:

Immunochemical evidence for the catalysis of vitamin D3 25-hydroxylation and testosterone 16 alpha-hydroxylation by homologous forms of cytochrome P-450 in rat liver microsomes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 103

Page Numbers: 853-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1988

Immunochemical evidence for the catalysis of vitamin D3 25-hydroxylation and testosterone 16 alpha-hydroxylation by homologous forms of cytochrome P-450 in rat liver microsomes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Immunochemical evidence for the catalysis of vitamin D3 25-hydroxylation and testosterone 16 alpha-hydroxylation by homologous forms of cytochrome P-450 in rat liver microsomes. Keywords Mesh Terms:

KEYWORDS: Steroid Hydroxylases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Immunochemical evidence for the catalysis of vitamin D3 25-hydroxylation and testosterone 16 alpha-hydroxylation by homologous forms of cytochrome P-450 in rat liver microsomes. Information

Substance Name: testosterone 7-alpha-hydroxylase, hamste

Registry Number: EC 1.14.14.1

Grant and Affiliation Information for Immunochemical evidence for the catalysis of vitamin D3 25-hydroxylation and testosterone 16 alpha-hydroxylation by homologous forms of cytochrome P-450 in rat liver microsomes.

AFFILIATION: Department of Biochemistry, School of Dentistry, Hiroshima University.

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Immunochemical evidence for the catalysis of vitamin D3 25-hydroxylation and testosterone 16 alpha-hydroxylation by homologous forms of cytochrome P-450 in rat liver microsomes Related Publications

• An improved mutarotase assay using beta-D-glucose oxidase and an oxygen electrode.
• Asymmetric dimethylarginine may be a missing link between cardiovascular disease and chronic kidney disease (Review Article).
• Cell wall extensibility during branch formation in the xanthophycean alga Vaucheria terrestris.
• Chimeric adenovirus type 5/35 vector encoding SIV gag and HIV env genes affords protective immunity against the simian/human immunodeficiency virus in monkeys.
• Correction of the darkened anterior tooth: a new CLEAR approach to an old problem.
• Effects of alpha- and beta-D-glucose on germination of spores of Bacillus megaterium QM B1551.
• Effects of free fatty acids on fibrinolytic activity.
• Expressions of estrogen receptors in the bovine corpus luteum: cyclic changes and effects of prostaglandin F2alpha and cytokines.
• Faraday instability of superfluid surface.
• Fas-mediated apoptosis is suppressed by calf serum in cultured bovine luteal cells.
• Fatty acid alcohol ester-synthesizing activity of lipoprotein lipase.
• Glucose requirement at different developmental stages of in vitro fertilized bovine embryos cultured in semi-defined medium.
• Identification of acylcholine acyl-hydrolase with carboxylic ester-hydrolase in human serum.
• In vitro penetration of zona pellucida of salt-stored bovine oocytes before and after maturation by frozen-thawed spermatozoa.
• Inhibitory action of tetracyclines on polynucleotide phosphorylase.
• Inhibitory effect of cranberry polyphenol on biofilm formation and cysteine proteases of Porphyromonas gingivalis.
• Lutetium alkyl and hydride complexes in a non-cyclopentadienyl coordination environment.
• Mesangiolytic glomerulopathy after radiotherapy and chemotherapy of gastric lymphoma.
• Partial synthesis of stero-bile acids related to chenodeoxycholic acid.
• Pigment epithelium-derived factor (PEDF) prevents advanced glycation end products (AGEs)-elicited endothelial nitric oxide synthase (eNOS) reduction through its anti-oxidative properties.
• Procyanidins in Callus and Multiple Shoot Cultures of Hypericum erectum1.
• Professional oral care reduces influenza infection in elderly.
• Prospection or projection: Neurobiological basis of stimulus-independent mental traveling.
• Reconstruction of chronic achilles tendon rupture with the use of interposed tissue between the stumps.
• Species-related differences in the mechanism of apoptosis during structural luteolysis.
• Stereochemical studies of hydrogen incorporation from nucleotides with fatty acid synthetase from Brevibacterium ammoniagenes.
• Suppression methods of radon emanation from phosphorus fertiliser and diatomaceous earth.
• [Medical practice in allergic diseases in the present Japanese medical health care system from the aspect of medical sociology]
• [The large intestine: examination methods and colon tumors]
• [The large intestine: inflammatory bowel disease (IBD)]