Immunochemical and histochemical studies on a phosphonoglycosphingolipid, SGL-II, isolated from the sea gastropod Aplysia kurodai.

Immunochemical and histochemical studies on a phosphonoglycosphingolipid, SGL-II, isolated from the sea gastropod Aplysia kurodai. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Immunochemical and histochemical studies on a phosphonoglycosphingolipid, SGL-II, isolated from the sea gastropod Aplysia kurodai. Abstract Text:

S Abe,Y Watanabe,S Araki,T Kumanishi,M Satake,

Antiserum was raised against 3-O-MeGal beta 1----3GalNAc alpha 1----3[6' -O-(2-aminoethylphosphonyl)Gal alpha 1----2 (2-aminoethylphosphonyl----6)Gal beta 1----4Glc beta 1----1Ceramide (SGL-II) isolated from the skin of a mollusc, Aplysia kurodai. This antiserum reacted with SGL-II and other phosphonoglycosphingolipids of Aplysia such as SGL-I', F-21, and some minor glycolipids on TLC plates, but it did not react with ganglioside or globoside. The sugars recognized were 3-O-methylgalactose at the non-reducing end and galactose at the branched chain of the glycolipids. One membrane glycoprotein (Mr 280,000) reacted strongly, and some other proteins reacted weakly with the antiserum. Immunohistochemical examination of the nervous tissues revealed distinct staining in the periganglionic tissue of the ganglia, and the perineural sheath of the proximal portion of the peripheral nerves. The neuropil and satellite cells were also stained. In the skin, subcutaneous connective tissues were moderately stained, and the cytoplasm of small mononuclear cells and foamy cells was also stained. The staining patterns were essentially the same in paraffin and cryostat sections. From the findings with sections pretreated with chloroform-methanol (2 : 1, v/v), it was suggested that the periganglionic and perineural stainings were due to glycoproteins, including an SDS-soluble glycoprotein of Mr 280,000, while those of the other regions were due to SGL-II and glycolipids immunologically related to SGL-II. The stainings in the skin sections were largely due to glycoproteins.

Immunochemical and histochemical studies on a phosphonoglycosphingolipid, SGL-II, isolated from the sea gastropod Aplysia kurodai. Publishing Authors By Initials

S Abe,Y Watanabe,S Araki,T Kumanishi,M Satake,

For similar integumentary system: skin research abstracts see: integumentary system: skin research

PUBMED ID PMID:

MEDLINE DATE:

Immunochemical and histochemical studies on a phosphonoglycosphingolipid, SGL-II, isolated from the sea gastropod Aplysia kurodai. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 104

Page Numbers: 220-6

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1988

Immunochemical and histochemical studies on a phosphonoglycosphingolipid, SGL-II, isolated from the sea gastropod Aplysia kurodai. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Immunochemical and histochemical studies on a phosphonoglycosphingolipid, SGL-II, isolated from the sea gastropod Aplysia kurodai. Keywords Mesh Terms:

KEYWORDS: Skin

MESH TERMS: analysis

Chemical & Substance for Abstract: Immunochemical and histochemical studies on a phosphonoglycosphingolipid, SGL-II, isolated from the sea gastropod Aplysia kurodai. Information

Substance Name: diphosphonopentaosylceramide

Registry Number: 103147-89-3

Grant and Affiliation Information for Immunochemical and histochemical studies on a phosphonoglycosphingolipid, SGL-II, isolated from the sea gastropod Aplysia kurodai.

AFFILIATION: Department of Neurochemistry, College of Biomedical Technology, Niigata University.

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Immunochemical and histochemical studies on a phosphonoglycosphingolipid, SGL-II, isolated from the sea gastropod Aplysia kurodai Related Publications

• 4-O-Acetyl mycarose. A new O-acetyl sugar obtained from leucomycin minor components.
• A SMAP Gene Family Encoding ARF GTPase-Activating Proteins and Its Implication in Membrane Trafficking.
• A novel sphingophosphonoglycolipid containing 3-O-methylgalactose isolated from the skin of the marine gastropod Aplysia kurodai.
• ACID AND ALKALINE DEGRADATION OF THE TNP-AMINO ACIDS AND -PEPTIDES.
• An enzyme cycling method for measurement of allantoin in human serum.
• Biochemical characterization of the neuron. ATPase and acetylcholinesterase activities of neuronal cell bodies isolated in bulk from the pig brain stem.
• Characterization of phosphonoglycosphingolipids containing pyruvate: localization in Aplysia nerve bundles.
• Chemical Constituents of Morinda citrifolia Roots Exhibit Hypoglycemic Effects in Streptozotocin-Induced Diabetic Mice.
• Clathrin dependent endocytosis of E-cadherin is regulated by the Arf6GAP isoform SMAP1.
• Clinical management of autoimmune pancreatitis.
• Comparison of 30 immunity-related genes from the common marmoset with orthologues from human and mouse.
• Different stability of N- and C-domain of diferric ovotransferrin in urea and application to the determination of iron distribution between the two domains.
• Fluorescence Response of a 4-Trifluoroacetylaminophthalimide to Iodide Ions upon 254 nm Irradiation in MeCN.
• Functions of a GnRH receptor heterodimer of the ascidian, Ciona intestinalis. Short communication.
• Gangliosides in neuron.
• Glycolipids in the nervous tissue of invertebrate.
• HEMOGLOBINS FROM ERYTHROCYTES OF EEL, ANGUILLA JAPONICA.
• Heterogeneity of hemoglobin from Arca (Anadara satowi). Molecular weights and oxygen equilibria of Arca Hb I and II.
• Immunochemical and histochemical studies on a phosphonoglycosphingolipid, SGL-II, isolated from the sea gastropod Aplysia kurodai.
• Isolation and characterization of a novel 2-aminoethylphosphonyl-glycosphingolipid from the sea hare, Aplysia kurodai.
• Negative cooperativity of chicken ovotransferrin on Al(III)-binding.
• On the chemical structure of porcine globin alpha.
• On the molecular weight of hemoglobin from Anadara inflata at various pH.
• Selective modification of myosin SH1 with 1,2,4-trinitrobenzene. VIII. Thiols of myosin.
• Steady state characteristics of acclimated hydrogenotrophic methanogens on inorganic substrate in continuous chemostat reactors.
• Structural analysis of a novel triphosphonoglycosphingolipid from the egg of the sea hare, Aplysia kurodai.
• Studies on insulin. IV. On the amino acid composition of the glycyl chain of Bonito insulin-I.
• The spectrophotometric determination as trinitrophenyl derivatives of amino acids and peptides resolved on paper by electrophoresis and chromatography.
• [Calcium oxalate crystals in several kinds of Cinnamon bark]
• [Patterns of mediastinal and supraclavicular metastases in apical invasive lung cancer--importance of supraclavicular lymph node dissection]