Immunization with dendritic cells loaded with alpha-galactosylceramide at priming phase, but not at boosting phase, enhances cytotoxic T lymphocyte activity against infection by intracellular bacteria.

Immunization with dendritic cells loaded with alpha-galactosylceramide at priming phase, but not at boosting phase, enhances cytotoxic T lymphocyte activity against infection by intracellular bacteria. Research Abstract Details 

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Immunization with dendritic cells loaded with alpha-galactosylceramide at priming phase, but not at boosting phase, enhances cytotoxic T lymphocyte activity against infection by intracellular bacteria. Abstract Text:

Noriyuki Enomoto,Toshi Nagata,Takafumi Suda,Masato Uchijima,Yutaro Nakamura,Kingo Chida,Hirotoshi Nakamura,Yukio Koide,Noriyuki Enomoto,Toshi Nagata,Takafumi Suda,Masato Uchijima,Yutaro Nakamura,Kingo Chida,Hirotoshi Nakamura,Yukio Koide,Noriyuki Enomoto,Toshi Nagata,Takafumi Suda,Masato Uchijima,Yutaro Nakamura,Kingo Chida,Hirotoshi Nakamura,Yukio Koide,

We evaluated the effect of immunization with dendritic cells (DCs) pulsed with alpha-galactosylceramide (alphaGalCer) and listeriolysin O (LLO) 91-99 peptide, a dominant cytotoxic T lymphocyte (CTL) epitope of Listeria monocytogenes by observing the responses of specific CD8(+) T cells and in vivo CTL activity. DCs were pulsed with various combinations of alphaGalCer and LLO91-99 peptide and administered to BALB/c mice. Immunization with DCs pulsed with alphaGalCer and LLO91-99 at priming phase and with DCs pulsed with LLO91-99 alone at boosting phase induced stronger in vivo CTL activity, reduced the bacterial load in spleens of Listeria-challenged mice and augmented CD62L(+) CD8(+) central memory T cells compared with other immunization protocols. The blockade of interferon-gamma (IFN-gamma) at boosting phase reversed the induction of CD8(+) central memory T cells and reduced the bacterial load in spleens of Listeria-challenged mice immunized with DCs pulsed with alphaGalCer and LLO91-99 at both phases, suggesting that alphaGalCer at boosting phase has deleterious effects through IFN-gamma production. These results indicate that immunization with DCs pulsed with CTL epitope peptide together with alphaGalCer at priming phase, but not at boosting phase, is feasible for eliciting a specific CTL activity and protective immunity against infection of intracellular bacteria.

Immunization with dendritic cells loaded with alpha-galactosylceramide at priming phase, but not at boosting phase, enhances cytotoxic T lymphocyte activity against infection by intracellular bacteria. Publishing Authors By Initials

N Enomoto,T Nagata,T Suda,M Uchijima,Y Nakamura,K Chida,H Nakamura,Y Koide,N Enomoto,T Nagata,T Suda,M Uchijima,Y Nakamura,K Chida,H Nakamura,Y Koide,N Enomoto,T Nagata,T Suda,M Uchijima,Y Nakamura,K Chida,H Nakamura,Y Koide,

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Immunization with dendritic cells loaded with alpha-galactosylceramide at priming phase, but not at boosting phase, enhances cytotoxic T lymphocyte activity against infection by intracellular bacteria. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: FEMS immunology and medical microbiology

VOLUME: 51

Page Numbers: 350-62

Journal Abbreviation: FEMS Immunol. Med. Microbiol.

ISSN: 0928-8244

DAY: 29

MONTH: 08

YEAR: 2007

Immunization with dendritic cells loaded with alpha-galactosylceramide at priming phase, but not at boosting phase, enhances cytotoxic T lymphocyte activity against infection by intracellular bacteria. Information

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LANGUAGE: eng

NlmUniqueID: 9315554

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Grant and Affiliation Information for Immunization with dendritic cells loaded with alpha-galactosylceramide at priming phase, but not at boosting phase, enhances cytotoxic T lymphocyte activity against infection by intracellular bacteria.

AFFILIATION: Department of Internal Medicine, Second Division, Hamamatsu University School of Medicine, Hamamatsu, Japan.

Country: England

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MEDLINETA: FEMS Immunol Med Microbiol

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