[Immunization with dendritic cells infected with human AFP adenovirus vector effectively elicits immunity against mouse hepatocellular carcinomas]

[Immunization with dendritic cells infected with human AFP adenovirus vector effectively elicits immunity against mouse hepatocellular carcinomas] Research Abstract Details 

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[Immunization with dendritic cells infected with human AFP adenovirus vector effectively elicits immunity against mouse hepatocellular carcinomas] Abstract Text:

Xiao-hua Tan,Qing Zhu,Chang Liu,Xiu-li Liu,Xiao-ting Shao,Bing Wei,Xiao-hua Tan,Qing Zhu,Chang Liu,Xiu-li Liu,Xiao-ting Shao,Bing Wei,

OBJECTIVE: To investigate the effects of dendritic cells (DCs) infected with adenovirus vector encoding xenogeneic alpha-fetoprotein (AFP) on breaking the immune tolerance and induction of immunity against hepatocellular carcinomas. METHODS: Human and mouse alpha-fetoprotein full-length cDNA were cloned from human HepG2 and mouse Hepa 1 - 6 hepatoma cell lines, respectively, using RT-PCR, and then inserted into adenoviral shuttle vectors to construct Ad hAFP and Ad mAFP. Mice were immunized with Ad hAFP-infected DC and in vitro CTL activity against Hepa 1 - 6 cells was examined by standard (51)Cr release assay. Survival was studied of the immunized mice, with or without depletion of CD8+ or CD4+ T cells, inoculated with Hepa 1 - 6 mouse hepatoma cells. RESULTS: The lytic activity of CTL elicited by the Ad hAFP-infected DCs were much stronger than that by Ad mAFP-infected DCs. 80% of the Ad hAFP/DCs-immunized mice of the inoculated with 5 x 10(6) Hepa 1 - 6 hepatoma cells were still alive two months after inoculation. However, the Ad mAFP/DCs-immunized mice inoculated with 1 x 10(6) Hepa 1 - 6 cells were just 20% surviving two months later. Depletion of CD8+ or CD4+ T cells abolished such an antigen-specific immunity elicited by the DCs infected with Ad hAFP. CONCLUSION: Adenovirus vector-mediated xenogeneic AFP-infected DCs can effectively break the immune tolerance to hepatocellular carcinomas in an animal model and induce strong antigen-specific T cell response, which are dependent on CD8+ and CD4+ T cells.

[Immunization with dendritic cells infected with human AFP adenovirus vector effectively elicits immunity against mouse hepatocellular carcinomas] Publishing Authors By Initials

XH Tan,Q Zhu,C Liu,XL Liu,XT Shao,B Wei,XH Tan,Q Zhu,C Liu,XL Liu,XT Shao,B Wei,

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[Immunization with dendritic cells infected with human AFP adenovirus vector effectively elicits immunity against mouse hepatocellular carcinomas] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhonghua zhong liu za zhi [Chinese journal of onco

VOLUME: 28

Page Numbers: 13-6

Journal Abbreviation: Zhonghua Zhong Liu Za Zhi

ISSN: 0253-3766

DAY: 1

MONTH: Jan

YEAR: 2006

[Immunization with dendritic cells infected with human AFP adenovirus vector effectively elicits immunity against mouse hepatocellular carcinomas] Information

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LANGUAGE: chi

NlmUniqueID: 7910681

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AFFILIATION: Department of Hematology, General Hospital of Beijing Military Command, Beijing 100700, China. xiaohua_t@hotmail.com

Country: China

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MEDLINETA: Zhonghua Zhong Liu Za Zhi

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