Immunization of mice.

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Immunization of mice. Abstract Text:

S A Fuller,M Takahashi,J G Hurrell,S A Fuller,M Takahashi,J G Hurrell,

In the protocols in this unit, antigen is prepared for injection either by emulsifying an antigen solution with Freunds adjuvant or by homogenizing a polyacrylamide gel slice containing the protein antigen. Mice are immunized at 2- to 3-week intervals. Test bleeds are collected 7 days after each booster immunization to monitor serum antibody levels. Mice are chosen for hybridoma fusions when a sufficient antibody titer is reached.

Immunization of mice. Publishing Authors By Initials

SA Fuller,M Takahashi,JG Hurrell,SA Fuller,M Takahashi,JG Hurrell,

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PUBLICATION TYPE: Journal Article

Journal: Current protocols in molecular biology / edited by

VOLUME: Chapter 11

Page Numbers: Unit11.4

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ISSN: 1934-3647

DAY: 11

MONTH: May

YEAR: 2001

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LANGUAGE: eng

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AFFILIATION: ADI Diagnostics, Rexdale, Ontario.

Country: United States

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MEDLINETA: Curr Protoc Mol Biol

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