Immobilization of acetylcholinesterase and choline oxidase in/on pHEMA membrane for biosensor construction.

Immobilization of acetylcholinesterase and choline oxidase in/on pHEMA membrane for biosensor construction. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Immobilization of acetylcholinesterase and choline oxidase in/on pHEMA membrane for biosensor construction. Abstract Text:

F N Kok,F Bozoglu,V Hasirci,

In this study, acetylcholinesterase (AChE) and choline oxidase (ChO) were co-immobilized on poly(2-hydroxyethyl methacrylate) (pHEMA) membranes with the aim of using them in biosensor construction. pHEMA membranes were prepared with the addition of different salts in different HEMA: aqueous solution ratios and characterized in terms of porosity, thickness. permeability, and mechanical properties. Membranes prepared in the presence of SnCl4 were found to be superior in terms of porosity and permeability and were chosen as the immobilization matrix. Immobilization of the enzymes was achieved both by entrapment and surface attachment via epichlorohydrin (Epi) and Cibacron Blue F36A (CB) activation. The effect of immobilization on enzyme activity was evaluated by the comparison of Km and Vmax values for the free and immobilized bi-enzyme systems. The increase in Km was negligible (1.08-fold) for the bi-enzyme system upon immobilization on surface but was 2.12-fold upon entrapment. Specific activity of the free enzyme system was found to be 0.306 mV s(-1) microg(-1) ChO while it was 0.069 (4.43-fold decrease) for entrapped and 0.198 (1.54 fold decrease) for CB-Epi immobilized enzymes. The performance of immobilized enzymes in different buffer types, pH, and temperature conditions were evaluated. The best enzyme activity was obtained at pH 9.0. Activity of the enzymes was found to increase with increasing temperature (in the range 25-40 degrees C).

Immobilization of acetylcholinesterase and choline oxidase in/on pHEMA membrane for biosensor construction. Publishing Authors By Initials

FN Kok,F Bozoglu,V Hasirci,

For similar inorganic chemicals: tin compounds research abstracts see: inorganic chemicals: tin compounds research

PUBMED ID PMID:

MEDLINE DATE:

Immobilization of acetylcholinesterase and choline oxidase in/on pHEMA membrane for biosensor construction. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biomaterials science. Polymer edition

VOLUME: 12

Page Numbers: 1161-76

Journal Abbreviation:

ISSN: 0920-5063

DAY: 20

MONTH: 02

YEAR: 2001

Immobilization of acetylcholinesterase and choline oxidase in/on pHEMA membrane for biosensor construction. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9007393

Immobilization of acetylcholinesterase and choline oxidase in/on pHEMA membrane for biosensor construction. Keywords Mesh Terms:

KEYWORDS: Tin Compounds

MESH TERMS: chemistry

Chemical & Substance for Abstract: Immobilization of acetylcholinesterase and choline oxidase in/on pHEMA membrane for biosensor construction. Information

Substance Name: Acetylcholinesterase

Registry Number: EC 3.1.1.7

Grant and Affiliation Information for Immobilization of acetylcholinesterase and choline oxidase in/on pHEMA membrane for biosensor construction.

AFFILIATION: Middle East Technical University, Department of Biological Sciences and Biotechnology, Ankara, Turkey.

Country: Netherlands

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biomater Sci Polym Ed

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Immobilization of acetylcholinesterase and choline oxidase in/on pHEMA membrane for biosensor construction Related Publications

• A novel construct as a cell carrier for tissue engineering.
• Assessment of biodegradable controlled release rod systems for pain relief applications.
• Biodegradable polyhydroxyalkanoate implants for osteomyelitis therapy: in vitro antibiotic release.
• Chemical and topographical modification of PHBV surface to promote osteoblast alignment and confinement.
• Collagen-chondroitin sulfate-based PLLA-SAIB-coated rhBMP-2 delivery system for bone repair.
• Contact guidance enhances the quality of a tissue engineered corneal stroma.
• Cytotoxicity evaluation of gelatin sponges prepared with different cross-linking agents.
• EDC/NHS cross-linked collagen foams as scaffolds for artificial corneal stroma.
• Electrospun biodegradable nanofibrous mats for tissue engineering.
• Hydroxyapatite reinforced poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) based degradable composite bone plate.
• Immobilization of acetylcholinesterase and choline oxidase in/on pHEMA membrane for biosensor construction.
• In vivo pain relief effectiveness of an analgesic-anesthetic carrying biodegradable controlled release rod systems.
• Nanopatterned collagen tubes for vascular tissue engineering.
• Reduction of peritoneal adhesions by sustained and local administration of epidermal growth factor.
• Sequential growth factor delivery from complexed microspheres for bone tissue engineering.
• Tissue responses to molecularly reinforced polylactide-co-glycolide implants.