Imaging properties in two-photon excitation microscopy and effects of refractive-index mismatch in thick specimens.

Imaging properties in two-photon excitation microscopy and effects of refractive-index mismatch in thick specimens. Research Abstract Details 

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Imaging properties in two-photon excitation microscopy and effects of refractive-index mismatch in thick specimens. Abstract Text:

The detrimental effects of a refractive-index mismatch on the image formation in a two-photon microscope were investigated. Point-spread functions (PSF's) were recorded with an oil-immersion objective numerical aperture (NA) of 1.3 and a water-immersion objective NA of 1.2 in an aqueous sample at different depths. For the oil-immersion objective the enlargement of the PSF volume with increasing depth yields an axial and a lateral loss in resolution of approximately 380% and 160%, respectively, at a 90-mum depth in the sample. For the water-immersion objective no resolution decrease was found. Measurements on a thick aqueous biofilm sample shows the importance of matching the refractive index between immersion fluid and sample. With a good match, no loss in image resolution is observed.

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Imaging properties in two-photon excitation microscopy and effects of refractive-index mismatch in thick specimens. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Applied optics

VOLUME: 38

Page Numbers: 5995-6003

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ISSN: 0003-6935

DAY: 1

MONTH: Oct

YEAR: 1999

Imaging properties in two-photon excitation microscopy and effects of refractive-index mismatch in thick specimens. Information

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LANGUAGE: eng

NlmUniqueID: 247660

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Country: United States

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MEDLINETA: Appl Opt

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