IL-1beta stimulates rat cardiac fibroblast migration via MAP kinase pathways.

IL-1beta stimulates rat cardiac fibroblast migration via MAP kinase pathways. Research Abstract Details 

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IL-1beta stimulates rat cardiac fibroblast migration via MAP kinase pathways. Abstract Text:

M Darren Mitchell,Rebecca E Laird,R Dale Brown,Carlin S Long,

The pro-inflammatory cytokines interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) are elevated following acute myocardial infarction (MI) and have been implicated in the pathophysiology of cardiac disease progression. The cardiac fibroblast represents an important effector cell target for cytokine actions. In particular, cytokine-directed cardiac fibroblast migration is likely to impact both myocardial repair following acute MI and pathological myocardial remodeling in the progression to heart failure. In the present study, we examined the migratory response of neonatal rat cardiac fibroblasts to pro-inflammatory cytokines using modified Boyden chamber assays. On the basis of the knowledge of migration in other cell types, we hypothesized that members of the mitogen-activated protein kinase (MAPK) family may regulate this process. This possibility was addressed with the use of immunoblot detection of active phosphorylated MAPK species and pharmacological inhibitors for individual members of the MAPK cascades. IL-1beta stimulated robust and concentration-dependent increases in migration (maximum, 20-fold over control cells). TNF-alpha had lesser effect (fourfold increase over control). IL-6 did not induce migration. Activation of all three MAPK subfamilies (extracellular signal-regulated kinases, c-Jun NH(2)-terminal kinases, and p38) was shown to occur in response to cytokine stimulation. Fibroblast migration was attenuated by pharmacological inhibition of each MAPK subfamily. Understanding the regulation of cardiac fibroblast migration may provide insights in the search for therapies aimed at enhancing the functional nature of the remodeling process.

IL-1beta stimulates rat cardiac fibroblast migration via MAP kinase pathways. Publishing Authors By Initials

MD Mitchell,RE Laird,RD Brown,CS Long,

For similar enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-serine-threonine kinases: mitogen-activated protein kinases: p38 mitogen-activated protein kinases research abstracts see: enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-serine-threonine kinases: mitogen-activated protein kinases: p38 mitogen-activated protein kinases research

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IL-1beta stimulates rat cardiac fibroblast migration via MAP kinase pathways. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: American journal of physiology. Heart and circulat

VOLUME: 292

Page Numbers: H1139-47

Journal Abbreviation: Am. J. Physiol. Heart Circ. Ph

ISSN: 0363-6135

DAY: 3

MONTH: 11

YEAR: 2006

IL-1beta stimulates rat cardiac fibroblast migration via MAP kinase pathways. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901228

IL-1beta stimulates rat cardiac fibroblast migration via MAP kinase pathways. Keywords Mesh Terms:

KEYWORDS: p38 Mitogen-Activated Protein Kinases

MESH TERMS: metabolism

Chemical & Substance for Abstract: IL-1beta stimulates rat cardiac fibroblast migration via MAP kinase pathways. Information

Substance Name: p38 Mitogen-Activated Protein Kinases

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for IL-1beta stimulates rat cardiac fibroblast migration via MAP kinase pathways.

AFFILIATION: Division of Cardiology, B-139, University of Colorado Health Sciences Center, 4200 E. 9th Ave., Denver, CO 80262, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL-59428

ACRONYM: HL

MEDLINETA: Am J Physiol Heart Circ Physio

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