IFN-gamma production and degranulation are differentially regulated in response to stimulation in murine natural killer cells.

IFN-gamma production and degranulation are differentially regulated in response to stimulation in murine natural killer cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

IFN-gamma production and degranulation are differentially regulated in response to stimulation in murine natural killer cells. Abstract Text:

G Vahlne,S Becker,P Brodin,M H Johansson,G Vahlne,S Becker,P Brodin,M H Johansson,G Vahlne,S Becker,P Brodin,M H Johansson,

Activation of natural killer (NK) cells is induced via receptors like NKG2D, NKR-P1C and NKp46. This activation is balanced by interactions with inhibitory receptors. NK cell activation can lead to cytotoxicity mediated via polarized exocytosis of secretory lysosomes (degranulation) and interferon (IFN)-gamma production. We studied cell surface mobilization of a molecule present in secretory lysosomes, CD107a (LAMP-1), to monitor the relationship between degranulation of NK cells and their production of IFN-gamma at the single cell level. A comparison of responses in naive mouse NK cells and NK cells pre-activated with the type I interferon-inducer tilorone demonstrated a dramatic influence of pre-activation, allowing potent degranulation and IFN-gamma responses to NKG2D mediated stimulation that were not observed with naive NK cells. Degranulation and IFN-gamma production were performed by overlapping NK cell populations with generally higher frequencies of degranulating than IFN-gamma producing NK cells. An NK cell subset analysis based on expression of Mac-1 and CD27 revealed that immature NK cells (Mac-1(lo) CD27(hi)) are preferentially degranulating, Mac-1(hi) CD27(hi) cells perform both effector functions efficiently, while the most mature (Mac-1(hi) CD27(lo)) NK cells display reduced degranulation but with maintained IFN-gamma production.

IFN-gamma production and degranulation are differentially regulated in response to stimulation in murine natural killer cells. Publishing Authors By Initials

G Vahlne,S Becker,P Brodin,MH Johansson,G Vahlne,S Becker,P Brodin,MH Johansson,G Vahlne,S Becker,P Brodin,MH Johansson,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

IFN-gamma production and degranulation are differentially regulated in response to stimulation in murine natural killer cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Scandinavian journal of immunology

VOLUME: 67

Page Numbers: 1-11

Journal Abbreviation: Scand. J. Immunol.

ISSN: 1365-3083

DAY: 19

MONTH: 11

YEAR: 2007

IFN-gamma production and degranulation are differentially regulated in response to stimulation in murine natural killer cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 323767

IFN-gamma production and degranulation are differentially regulated in response to stimulation in murine natural killer cells. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: IFN-gamma production and degranulation are differentially regulated in response to stimulation in murine natural killer cells. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for IFN-gamma production and degranulation are differentially regulated in response to stimulation in murine natural killer cells.

AFFILIATION: Department for Microbiology, Tumor and Cell biology, Karolinska Institutet, Stockholm, Sweden.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Scand J Immunol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

IFN-gamma production and degranulation are differentially regulated in response to stimulation in murine natural killer cells Related Publications

• A review of the impact of photon and proton external beam radiotherapy treatment modalities on the dose distribution in field and out-of-field; implications for the long-term morbidity of cancer survivors.
• Anticipatory models in gaze control: a developmental model.
• Bleeding during transurethral prostatectomy. Is preoperative blood request necessary?
• Capsular and ligament injuries of the elbow joint. A clinical and arthrographic study.
• Changes in multi-segmented body movements and EMG activity while standing on firm and foam support surfaces.
• Choosing reporter-quencher pairs for efficient quenching through formation of intramolecular dimers.
• Combined photoplethysmographic monitoring of respiration rate and pulse: a comparison between different measurement sites in spontaneously breathing subjects.
• Differential engagement of modules 1 and 4 of vascular cell adhesion molecule-1 (CD106) by integrins alpha4beta1 (CD49d/29) and alphaMbeta2 (CD11b/18) of eosinophils.
• Differential locomotion of long- and short-term IL-2-activated murine natural killer cells in a model matrix environment.
• EGFR and beta1 integrins utilize different signaling pathways to activate Akt.
• End-to-end transureteroureterostomy in a patient with a single kidney. A case report.
• Fluorescence depolarization studies of filamentous actin analyzed with a genetic algorithm.
• Gastrointestinal application of laser Doppler flowmetry. An experimental and clinical study in cat and man.
• Gastrointestinal hormones.
• Higher Thrombelastograph platelet reactivity in cardiac surgery patients than in blood donors.
• IFN-gamma production and degranulation are differentially regulated in response to stimulation in murine natural killer cells.
• Impairment of the hemostatic potential of platelets during storage as evaluated by flow cytometry, thrombin generation, and thrombelastography under conditions promoting formation of coated platelets.
• Implications of bride price on domestic violence and reproductive health in Wakiso District, Uganda.
• Longitudinal changes in physical functional performance among the oldest old: insight from a study of Swedish twins.
• Modeling crowd turbulence by many-particle simulations.
• Mutations of FLT3, NRAS, KRAS, and PTPN11 are frequent and possibly mutually exclusive in high hyperdiploid childhood acute lymphoblastic leukemia.
• Physical, cognitive, and psychosocial variables from the disablement process model predict patterns of independence and the transition into disability for the oldest-old.
• Population inference with mortality and attrition in longitudinal studies on aging: a two-stage multiple imputation method.
• Studies of host-graft interactions in vitro.
• The Swedish rectal cancer registry.
• The eukaryotic leading and lagging strand DNA polymerases are loaded onto primer-ends via separate mechanisms but have comparable processivity in the presence of PCNA.
• The symmetry of two-photon excited states as determined by time-resolved fluorescence depolarization experiments.
• Thermography of the breast. A clinical study with special reference to breast cancer detection.
• Tiling resolution array comparative genomic hybridization, expression and methylation analyses of dup(1q) in Burkitt lymphomas and pediatric high hyperdiploid acute lymphoblastic leukemias reveal clustered near-centromeric breakpoints and overexpression o
• Tracheostomy inner cannula care: a randomized crossover study of two decontamination procedures.