Identifying genes differentially expressed between PGCs and ES cells reveals a role for CREB-binding protein in germ cell survival.

Identifying genes differentially expressed between PGCs and ES cells reveals a role for CREB-binding protein in germ cell survival. Research Abstract Details 

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Identifying genes differentially expressed between PGCs and ES cells reveals a role for CREB-binding protein in germ cell survival. Abstract Text:

Aaron M Elliott,Maria P de Miguel,Vivienne I Rebel,Peter J Donovan,Aaron M Elliott,Maria P de Miguel,Vivienne I Rebel,Peter J Donovan,

Primordial germ cells (PGCs) are the embryonic precursors of the adult gametes. Although restricted in developmental potency, PGCs express many of the same molecular markers as pluripotent embryonic stem (ES) cells and can give rise to embryonal carcinoma (EC) cells, the stem cells of testicular tumors, in vivo. Likewise, when exposed to specific growth factors in vitro PGCs can be converted into pluripotent embryonic germ (EG) cells. Here, we propose that genes differentially expressed between PGCs and ES cells are good candidates for regulating germline development. To identify genes important in regulating germ cell development and mammalian fertility, we performed suppression subtraction hybridization (SSH) between PGCs and ES cells whole gene set. Using this method, we identified the transcriptional coactivator/histone acetyltransferase CREB-binding protein (CBP) as being highly expressed in PGCs compared to ES cells. To elucidate the function of CBP in PGCs, we generated mice with a PGC-specific deletion of CBP. Loss of CBP in PGCs leads to increased apoptosis and subsequent reduction in PGC numbers. These data indicate an essential role for CBP in maintaining normal germ cell development.

Identifying genes differentially expressed between PGCs and ES cells reveals a role for CREB-binding protein in germ cell survival. Publishing Authors By Initials

AM Elliott,MP de Miguel,VI Rebel,PJ Donovan,AM Elliott,MP de Miguel,VI Rebel,PJ Donovan,

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Identifying genes differentially expressed between PGCs and ES cells reveals a role for CREB-binding protein in germ cell survival. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Developmental biology

VOLUME: 311

Page Numbers: 347-58

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ISSN: 1095-564X

DAY: 22

MONTH: 08

YEAR: 2007

Identifying genes differentially expressed between PGCs and ES cells reveals a role for CREB-binding protein in germ cell survival. Information

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LANGUAGE: eng

NlmUniqueID: 372762

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Grant and Affiliation Information for Identifying genes differentially expressed between PGCs and ES cells reveals a role for CREB-binding protein in germ cell survival.

AFFILIATION: Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA.

Country: United States

AGENCY: United States NICHD

GRANT: R03 HD41511

ACRONYM: HD

MEDLINETA: Dev Biol

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