Identifying Chromosomal Targets of DNA-Binding Proteins by Sequence Tag Analysis of Genomic Enrichment (STAGE).

Identifying Chromosomal Targets of DNA-Binding Proteins by Sequence Tag Analysis of Genomic Enrichment (STAGE). Research Abstract Details 

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Identifying Chromosomal Targets of DNA-Binding Proteins by Sequence Tag Analysis of Genomic Enrichment (STAGE). Abstract Text:

Jonghwan Kim,Vishwanath R Iyer,Jonghwan Kim,Vishwanath R Iyer,Jonghwan Kim,Vishwanath R Iyer,

Sequence Tag Analysis of Genomic Enrichment (STAGE) is a method for experimentally identifying the in vivo chromosomal targets of DNA-binding proteins in any sequenced genome. STAGE generates 21-bp tags derived from DNA isolated by chromatin immunoprecipitation (ChIP; UNIT 21.3). Concatamers of tags are cloned and sequenced to yield a STAGE library. Tags in the library represent DNA fragments that were occupied by the DNA-binding protein, and mapping these tag sequences to the genome identifies the binding loci of the DNA-binding protein in vivo. STAGE can be applied to any sequenced genome to identify targets of DNA-binding proteins without requiring extensive microarray resources.

Identifying Chromosomal Targets of DNA-Binding Proteins by Sequence Tag Analysis of Genomic Enrichment (STAGE). Publishing Authors By Initials

J Kim,VR Iyer,J Kim,VR Iyer,J Kim,VR Iyer,

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Identifying Chromosomal Targets of DNA-Binding Proteins by Sequence Tag Analysis of Genomic Enrichment (STAGE). Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Current protocols in molecular biology / edited by

VOLUME: Chapter 21

Page Numbers: Unit21.10

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ISSN: 1934-3647

DAY: 11

MONTH: Nov

YEAR: 2005

Identifying Chromosomal Targets of DNA-Binding Proteins by Sequence Tag Analysis of Genomic Enrichment (STAGE). Information

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LANGUAGE: eng

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AFFILIATION: University of Texas at Austin, Austin, Texas.

Country: United States

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MEDLINETA: Curr Protoc Mol Biol

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