Identification of unique genetic markers in Rv0927c among Mycobacterium tuberculosis W-Beijing strains.

Identification of unique genetic markers in Rv0927c among Mycobacterium tuberculosis W-Beijing strains. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Identification of unique genetic markers in Rv0927c among Mycobacterium tuberculosis W-Beijing strains. Abstract Text:

Xin Jiang,Ying Zhang,Wenhong Zhang,Feng Gao,Jialin Jin,Xuelian Zhang,Chong Lv,Qian Gao,Jing Chen,Honghai Wang,

Fifty-six clinical isolates of Mycobacterium tuberculosis were analyzed by spoligotyping to determine the prevalence of W-Beijing strains. Forty-nine of the 56 isolates belonged to W-Beijing strains and 7 isolates were non-Beijing strains. Comparative two-dimensional gel electrophoresis analysis of protein patterns between the W-Beijing and non-Beijing strains identified a unique protein Rv0927c that is absent in the former but present in the latter and the reference strain M. tuberculosis H37Rv. Compared with 7 non-Beijing clinical isolates and H37Rv, all 49 W-Beijing strains had two characteristic mutations, a deletion of AGC at nucleotide position 421 of Rv0927c gene encoding a putative short dehydrogenase/reductase, causing deletion of serine codon at amino acid position 141 and a -127 G-->A mutation in Rv0927c-pstS3 intergenic region, resulting in failure to express Rv0927c. Western blot analysis indicated that polyclonal antibody raised against H37Rv Rv0927c overexpressed in Escherichia coli reacted with non-Beijing strains and H37Rv but not W-Beijing strains. Characteristic mutations of Rv0927c that are present in W-Beijing strains can be used as a novel genetic marker for rapid molecular typing of M. tuberculosis W-Beijing strains.

Identification of unique genetic markers in Rv0927c among Mycobacterium tuberculosis W-Beijing strains. Publishing Authors By Initials

X Jiang,Y Zhang,W Zhang,F Gao,J Jin,X Zhang,C Lv,Q Gao,J Chen,H Wang,

For similar bacterial infections and mycoses: bacterial infections: gram-positive bacterial infections: actinomycetales infections: mycobacterium infections: tuberculosis research abstracts see: bacterial infections and mycoses: bacterial infections: gram-positive bacterial infections: actinomycetales infections: mycobacterium infections: tuberculosis research

PUBMED ID PMID:

MEDLINE DATE:

Identification of unique genetic markers in Rv0927c among Mycobacterium tuberculosis W-Beijing strains. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Microbes and infection / Institut Pasteur

VOLUME: 9

Page Numbers: 241-6

Journal Abbreviation: Microbes Infect.

ISSN: 1286-4579

DAY: 18

MONTH: 12

YEAR: 2006

Identification of unique genetic markers in Rv0927c among Mycobacterium tuberculosis W-Beijing strains. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100883508

Identification of unique genetic markers in Rv0927c among Mycobacterium tuberculosis W-Beijing strains. Keywords Mesh Terms:

KEYWORDS: Tuberculosis

MESH TERMS: microbiology

Chemical & Substance for Abstract: Identification of unique genetic markers in Rv0927c among Mycobacterium tuberculosis W-Beijing strains. Information

Substance Name: Oxidoreductases

Registry Number: EC 1.-

Grant and Affiliation Information for Identification of unique genetic markers in Rv0927c among Mycobacterium tuberculosis W-Beijing strains.

AFFILIATION: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, PR China.

Country: France

AGENCY: United States NIAID

GRANT: AI44063

ACRONYM: AI

MEDLINETA: Microbes Infect

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Identification of unique genetic markers in Rv0927c among Mycobacterium tuberculosis W-Beijing strains Related Publications

• A novel link between the proteasome pathway and the signal transduction pathway of the bone morphogenetic proteins (BMPs).
• Effects of phytoestrogen genistein on delayed after depolarization and triggered activity induced by ouabain in guinea pig papillary muscles.
• Occupational health in China.
• Research on expression of telomerase in human odontogenic lesions.
• Suppression of tumor necrosis factor-mediated apoptosis by nuclear factor kappaB-independent bone morphogenetic protein/Smad signaling.
• [A gene analysis of the low density lipoprotein receptor in Chinese with homozygous familial hyperchol-esterolemia]
• [A novel rat aplastic anemia model induced by 5-fluorouracil combined with busulfan]
• [A study of association between atherothrombotic brain infarction and HLA-DQA1 peptide binding motifs]
• [An experimental study on the bone repairing ability of recombinant human bone morphogenetic protein-2-coral composited artificial bone]
• [Analysis of EEG based on the complexity measure]
• [Apoptosis of bone marrow cells aplastic anemia patients treated with cyclosporin A]
• [Carrier detection and prenatal diagnosis for hemophilia A]
• [Changes of the expression of integrin alpha6beta1 and the activity of focal adhesion kinase in the liver sinusoidal endothelial cell in experimental fibrotic rats]
• [Clinical analysis of 22 patients with primary biliary cirrhosis]
• [Clinical significance of hemostatic molecular markers in acute leukemia]
• [Effects of phenytoin on structural aberration of human sperm chromosomes in vitro]
• [Expression and purification of human papillomavirus type16 L1 protein in a prokaryotic expression system]
• [Extension of cardiac monitoring function by used of ordinary ECG machine]
• [Histopathological features of 27 cases of primary biliary cirrhosis]
• [Multiagent system for individual artificial hip joint]
• [Neomycin inhibits nicotinic receptor-mediated response in pheochromocytoma (PC12) cells]
• [No evidence for association between the alpha 2-macroglobulin polymorphism and Alzheimer's disease in the Han Chinese]
• [PCR cloning of 3'-terminal repeat fragments of Clostridium difficile toxin A and toxin B gene]
• [Preliminary study of prevention from restenosis of hepatic venous in stents by gene transfer of phVEGF165]
• [Prostaglandin inhibitor indomethacin inhibits afferent activities of Adelta and C units in the saphenous nerve of diabetic hyperalgesic rats]
• [Research and development on microwave sterilization]
• [Role of recombinant human bone morphogenetic protein 2/collagen as an onlay bone graft on adult rat calvarial bone]
• [Study of telomerase activity and its related genes expression in leukemia cells]
• [Thrombopoietic effect of recombinant human thrombopoietin gene transferred to mice mediated by electric pulse on normal and experimental thrombocytopenia mice]
• [Thymectomy for late-onset myasthenia gravis in the elderly (with report of 23 cases)]