Identification of tubulin as a substrate of Jak2 tyrosine kinase and its role in Jak2-dependent signaling.

Identification of tubulin as a substrate of Jak2 tyrosine kinase and its role in Jak2-dependent signaling. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Identification of tubulin as a substrate of Jak2 tyrosine kinase and its role in Jak2-dependent signaling. Abstract Text:

Xianyue Ma,Peter P Sayeski,

Jak2 is a nonreceptor tyrosine kinase that acts in numerous cellular signal transduction systems. Here, large amounts of recombinant Jak2 protein were expressed in eukaryotic cells, and an unknown 55 kDa protein was copurified with it. Mass spectrometry and Western blot analysis identified the 55 kDa protein as the alpha- and beta-isoforms of tubulin. Biochemical experiments determined that Jak2 and tubulin specifically coassociate with one another, and the region of Jak2 that binds tubulin is the pseudokinase domain. Immunofluoresence indicated that Jak2 and tubulin (microtubules) colocalize within intact cells. The functional consequence of the coassociation between Jak2 and tubulin is that Jak2 phosphorylates tubulin on tyrosine residues. Specifically, in response to growth hormone, tubulin was phosphorylated in a Jak2-dependent manner. Tubulin was also found to interact with signal transducers and activators of transcription 1 (STAT1) and be involved in STAT1 nuclear transport. As such, this work suggests that tubulin is a substrate of Jak2 and facilitates Jak2/STAT1-dependent signaling.

Identification of tubulin as a substrate of Jak2 tyrosine kinase and its role in Jak2-dependent signaling. Publishing Authors By Initials

X Ma,PP Sayeski,

For similar amino acids, peptides, and proteins: amino acids: amino acids, cyclic: amino acids, aromatic: tyrosine research abstracts see: amino acids, peptides, and proteins: amino acids: amino acids, cyclic: amino acids, aromatic: tyrosine research

PUBMED ID PMID:

MEDLINE DATE:

Identification of tubulin as a substrate of Jak2 tyrosine kinase and its role in Jak2-dependent signaling. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochemistry

VOLUME: 46

Page Numbers: 7153-62

Journal Abbreviation: Biochemistry

ISSN: 0006-2960

DAY: 26

MONTH: 05

YEAR: 2007

Identification of tubulin as a substrate of Jak2 tyrosine kinase and its role in Jak2-dependent signaling. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370623

Identification of tubulin as a substrate of Jak2 tyrosine kinase and its role in Jak2-dependent signaling. Keywords Mesh Terms:

KEYWORDS: Tyrosine

MESH TERMS: chemistry

Chemical & Substance for Abstract: Identification of tubulin as a substrate of Jak2 tyrosine kinase and its role in Jak2-dependent signaling. Information

Substance Name: Janus Kinase 2

Registry Number: EC 2.7.1.112

Grant and Affiliation Information for Identification of tubulin as a substrate of Jak2 tyrosine kinase and its role in Jak2-dependent signaling.

AFFILIATION: Department of Physiology and Functional Genomics, University of Florida College of Medicine, P.O. Box 100274, Gainesville, Florida 32610, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: R01-HL67277

ACRONYM: HL

MEDLINETA: Biochemistry

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Identification of tubulin as a substrate of Jak2 tyrosine kinase and its role in Jak2-dependent signaling Related Publications

• Identification of tubulin as a substrate of Jak2 tyrosine kinase and its role in Jak2-dependent signaling.
• Identification of tyrosine 972 as a novel site of Jak2 tyrosine kinase phosphorylation and its role in Jak2 activation.
• Jak2 tyrosine kinase and cancer: how good cells get HiJAKed.
• Jak2 tyrosine kinase mediates oxidative stress-induced apoptosis in vascular smooth muscle cells.
• Jak2 tyrosine kinase: a true jak of all trades?
• Z3, a novel Jak2 tyrosine kinase small-molecule inhibitor that suppresses Jak2-mediated pathologic cell growth.