Identification of trypsin inhibitor in bovine pituitary extracts as a survival factor for adult rat hepatocytes in primary culture.

Identification of trypsin inhibitor in bovine pituitary extracts as a survival factor for adult rat hepatocytes in primary culture. Research Abstract Details 

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Identification of trypsin inhibitor in bovine pituitary extracts as a survival factor for adult rat hepatocytes in primary culture. Abstract Text:

O Asami,T Nakamura,T Mura,A Ichihara,

Adult rat hepatocytes in primary culture cannot survive more than 2 days in the absence of calf serum. An extract of bovine pituitary gland had a similar effect to calf serum on the survival of hepatocytes, but its specific activity was 70 times that of calf serum. The survival factor was purified from bovine pituitary gland, and obtained in a homogeneous state judging by sodium dodecylsulfate-polyacrylamide gel electrophoresis and high performance liquid chromatography. Its purification was achieved by acid extraction of the pituitary gland, gel filtrations on Sephadex G-75 and Ultrogel AcA 202, ion-exchanger chromatography on CM-cellulose, and then reverse phase high performance liquid chromatography. The purified factor was effective at 10 ng/ml and maximally effective at 100 ng/ml. The overall recovery of its activity was 30%, and the specific activity of the purified factor was 3,100 times that in the acid extract. The molecular weight (Mr 8,000), estimated by Sephadex G-200 filtration, and the strong basic character (pI 10.5) of the purified survival factor suggested that it may be the trypsin inhibitor found in various tissues. Indeed, the amino acid composition of the pure material was identical with that of bovine pancreatic trypsin inhibitor (bPTI). The purified survival factor had the same inhibitory activity on trypsin as commercial bPTI and, conversely, commercial bPTI greatly enhanced survival of rat hepatocytes. Thus, the survival factor in bovine pituitary gland was identified as bPTI.

Identification of trypsin inhibitor in bovine pituitary extracts as a survival factor for adult rat hepatocytes in primary culture. Publishing Authors By Initials

O Asami,T Nakamura,T Mura,A Ichihara,

For similar chemical actions and uses: pharmacologic actions: molecular mechanisms of pharmacological action: enzyme inhibitors: protease inhibitors: serine proteinase inhibitors: trypsin inhibitors research abstracts see: chemical actions and uses: pharmacologic actions: molecular mechanisms of pharmacological action: enzyme inhibitors: protease inhibitors: serine proteinase inhibitors: trypsin inhibitors research

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Identification of trypsin inhibitor in bovine pituitary extracts as a survival factor for adult rat hepatocytes in primary culture. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 95

Page Numbers: 299-309

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1984

Identification of trypsin inhibitor in bovine pituitary extracts as a survival factor for adult rat hepatocytes in primary culture. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Identification of trypsin inhibitor in bovine pituitary extracts as a survival factor for adult rat hepatocytes in primary culture. Keywords Mesh Terms:

KEYWORDS: Trypsin Inhibitors

MESH TERMS: physiology

Chemical & Substance for Abstract: Identification of trypsin inhibitor in bovine pituitary extracts as a survival factor for adult rat hepatocytes in primary culture. Information

Substance Name: Fatty Acid Desaturases

Registry Number: EC 1.14.99.-

Grant and Affiliation Information for Identification of trypsin inhibitor in bovine pituitary extracts as a survival factor for adult rat hepatocytes in primary culture.

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Country: JAPAN

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MEDLINETA: J Biochem

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