Identification of the HIV-1 gp41 core-binding motif in the scaffolding domain of caveolin-1.

Identification of the HIV-1 gp41 core-binding motif in the scaffolding domain of caveolin-1. Research Abstract Details 

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Identification of the HIV-1 gp41 core-binding motif in the scaffolding domain of caveolin-1. Abstract Text:

Jing-He Huang,Lu Lu,Hong Lu,Xi Chen,Shibo Jiang,Ying-Hua Chen,

The human immunodeficiency virus, type 1 (HIV-1) gp41 core plays an important role in fusion between viral and target cell membranes. A single chain polypeptide, N36(L8)C34, which forms a six-helix bundle in physiological solution, can be used as a model of gp41 core. Here we identified from a 12-mer phage peptide library a positive phage clone displaying a peptide sequence with high binding activity to the HIV-1 gp41 core. The peptide sequence contains a putative gp41-binding motif, PhiXXXXPhiXPhi (X is any amino acid residue, and Phi is any one of the aromatic amino acid residues Trp, Phe, or Tyr). This motif also exists in the scaffolding domain of caveolin-1 (Cav-1), a known gp41-binding protein. Cav-1-(61-101) and Cav-1-(82-101), two recombinant fusion proteins containing the Cav-1 scaffolding domain, bound significantly to the gp41 expressed in mammalian cells and interacted with the polypeptide N36(L8)C34. These results suggest that the scaffolding domain of Cav-1 may bind to the gp41 core via the motif. This interaction may be essential for formation of fusion pore or endocytosis of HIV-1 and affect the pathogenesis of HIV-1 infection. Further characterization of the gp41 core-binding motifs may shed light on the alternative mechanism by which HIV-1 enters into the target cell.

Identification of the HIV-1 gp41 core-binding motif in the scaffolding domain of caveolin-1. Publishing Authors By Initials

JH Huang,L Lu,H Lu,X Chen,S Jiang,YH Chen,

For similar proteins: recombinant proteins: recombinant fusion proteins research abstracts see: proteins: recombinant proteins: recombinant fusion proteins research

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Identification of the HIV-1 gp41 core-binding motif in the scaffolding domain of caveolin-1. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 6143-52

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 31

MONTH: 12

YEAR: 2006

Identification of the HIV-1 gp41 core-binding motif in the scaffolding domain of caveolin-1. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Identification of the HIV-1 gp41 core-binding motif in the scaffolding domain of caveolin-1. Keywords Mesh Terms:

KEYWORDS: Recombinant Fusion Proteins

MESH TERMS: physiology

Chemical & Substance for Abstract: Identification of the HIV-1 gp41 core-binding motif in the scaffolding domain of caveolin-1. Information

Substance Name: Recombinant Fusion Proteins

Registry Number: 0

Grant and Affiliation Information for Identification of the HIV-1 gp41 core-binding motif in the scaffolding domain of caveolin-1.

AFFILIATION: Laboratory of Immunology, Department of Biology, Tsinghua University, Protein Science Laboratory of the Ministry of Education, Beijing 100084, P.R. China.

Country: United States

AGENCY: United States NIAID

GRANT: AI46221

ACRONYM: AI

MEDLINETA: J Biol Chem

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