Identification of the functional periplasmic nitrate reductase (nap) gene cluster from the deep-sea denitrifier Pseudomonas sp. strain MT-1.

Identification of the functional periplasmic nitrate reductase (nap) gene cluster from the deep-sea denitrifier Pseudomonas sp. strain MT-1. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Identification of the functional periplasmic nitrate reductase (nap) gene cluster from the deep-sea denitrifier Pseudomonas sp. strain MT-1. Abstract Text:

Hideyuki Tamegai,Eriko Ikeda,Chiaki Kato,Koki Horikoshi,Hideyuki Tamegai,Eriko Ikeda,Chiaki Kato,Koki Horikoshi,

The nap gene cluster encoding periplasmic nitrate reductase was identified from Pseudomonas sp. strain MT-1, a deep-sea denitrifier isolated from the Mariana Trench. The ORFs identified were highly homologous with those of Pseudomonas stutzeri, but the cluster included only four ORFs (napDABC), less than those in other organisms. For other bacteria, some additional small ORFs (such as napE, napF, napG, napH, and napK) are found in the nap gene cluster, although their physiological function is still unclear. The soluble fraction of MT-1 grown under denitrifying condition showed significant nitrate reductase activity. This observation suggests that the periplasmic nitrate reductase encoded by the gene cluster identified in this study is functional. The activity was highest when the organism was grown under denitrifying conditions, suggesting that the enzyme participates in dissimilatory nitrite reduction.

Identification of the functional periplasmic nitrate reductase (nap) gene cluster from the deep-sea denitrifier Pseudomonas sp. strain MT-1. Publishing Authors By Initials

H Tamegai,E Ikeda,C Kato,K Horikoshi,H Tamegai,E Ikeda,C Kato,K Horikoshi,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Identification of the functional periplasmic nitrate reductase (nap) gene cluster from the deep-sea denitrifier Pseudomonas sp. strain MT-1. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Bioscience, biotechnology, and biochemistry

VOLUME: 71

Page Numbers: 2041-5

Journal Abbreviation: Biosci. Biotechnol. Biochem.

ISSN: 0916-8451

DAY: 7

MONTH: 08

YEAR: 2007

Identification of the functional periplasmic nitrate reductase (nap) gene cluster from the deep-sea denitrifier Pseudomonas sp. strain MT-1. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9205717

Identification of the functional periplasmic nitrate reductase (nap) gene cluster from the deep-sea denitrifier Pseudomonas sp. strain MT-1. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Identification of the functional periplasmic nitrate reductase (nap) gene cluster from the deep-sea denitrifier Pseudomonas sp. strain MT-1. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Identification of the functional periplasmic nitrate reductase (nap) gene cluster from the deep-sea denitrifier Pseudomonas sp. strain MT-1.

AFFILIATION: Department of Chemistry, College of Humanities and Sciences, Nihon University, Sakurajosui, Tokyo, Japan. htamegai@chs.nihon-u.ac.jp

Country: Japan

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Biosci Biotechnol Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Identification of the functional periplasmic nitrate reductase nap gene cluster from the deep-sea denitrifier Pseudomonas sp strain MT-1 Related Publications

• Cell proliferation at 122{degrees}C and isotopically heavy CH4 production by a hyperthermophilic methanogen under high-pressure cultivation.
• Characterization of histamine release in digitonin-permeabilized rabbit platelets.
• Cloning of leucine genes of alkalophilic Bacillus No. 221 in E. coli and B. subtilis.
• Comparison of the gene expression of aspartate beta-D-semialdehyde dehydrogenase at elevated hydrostatic pressure in deep-sea bacteria.
• Culture-Dependent and -Independent Characterization of Microbial Communities Associated with a Shallow Submarine Hydrothermal System Occurring within a Coral Reef off Taketomi Island, Japan.
• Desulfothermus okinawensis sp. nov., a thermophilic and heterotrophic sulfate-reducing bacterium isolated from a deep-sea hydrothermal field.
• Differential scanning calorimetry study on the inner membrane lipids prepared from barotolerant Pseudomonas sp. BT1.
• Dipodascus tetrasporeus sp. nov., an ascosporogenous yeast isolated from deep-sea sediments in the Japan Trench.
• Exilispira thermophila gen. nov., sp. nov., an anaerobic, thermophilic spirochaete isolated from a deep-sea hydrothermal vent chimney.
• Further properties of sodium ion-stimulated alpha-[1-14C]aminoisobutyric acid uptake in alkalophilic Bacillus species.
• Histone chaperones: 30 years from isolation to elucidation of the mechanisms of nucleosome assembly and disassembly.
• Hydrogenivirga okinawensis sp. nov., a thermophilic sulfur-oxidizing chemolithoautotroph isolated from a deep-sea hydrothermal field, Southern Okinawa Trough.
• Identification of the functional periplasmic nitrate reductase (nap) gene cluster from the deep-sea denitrifier Pseudomonas sp. strain MT-1.
• Immobilized cyclomaltodextrin glucanotransferase of an alkalophilic Bacillus sp. No. 38-2.
• Isolation and initial characterization of GBF, a novel DNA-binding zinc finger protein that binds to the GC-rich binding sites of the HIV-1 promoter.
• Microbulbifer agarilyticus sp. nov. and Microbulbifer thermotolerans sp. nov., agar-degrading bacteria isolated from deep-sea sediment.
• Neptunomonas japonica sp. nov., an Osedax japonicus symbiont-like bacterium isolated from sediment adjacent to sperm whale carcasses off Kagoshima, Japan.
• Nucleotide and deduced amino acid sequences of a subtilisin-like serine protease from a deep-sea bacterium, Alkalimonas collagenimarina AC40(T).
• Nucleotide sequence of the glutamine synthetase gene (glnA) and its upstream region from Bacillus cereus.
• Open reading frame 3 of the barotolerant bacterium strain DSS12 is complementary with cydD in Escherichia coli: cydD functions are required for cell stability at high pressure.
• Oxidative Stress Plays a Critical Role in Inactivating Mutant BRAF by Geldanamycin Derivatives.
• Past, present and future of extremophiles.
• Phylogenetic and enzymatic diversity of deep subseafloor aerobic microorganisms in organics- and methane-rich sediments off Shimokita Peninsula.
• Properties of glutamate dehydrogenase and its involvement in alanine production in a hyperthermophilic archaeon, Thermococcus profundus.
• Psychromonas japonica sp. nov., Psychromonas aquimarina sp. nov., Psychromonas macrocephali sp. nov. and Psychromonas ossibalaenae sp. nov., psychrotrophic bacteria isolated from sediment adjacent to sperm whale carcasses off Kagoshima, Japan.
• Purification and properties of a cytochrome b560-d complex, a terminal oxidase of the aerobic respiratory chain of Photobacterium phosphoreum.
• Relationship between the subcellular localization and structures of catalytic domains of FKBP-type PPIases.
• Restricted expression of a member of the transcription elongation factor S-II family in testicular germ cells during and after meiosis.
• Sodium-ion stimulated amino acid uptake in membrane vesicles of alkalophilic Bacillus no. 8-1.
• The involvement of the histone fold motifs in the mutual interaction between human TAF(II)80 and TAF(II)22.