Identification of the core transmembrane complex of the Legionella Dot/Icm type IV secretion system.

Identification of the core transmembrane complex of the Legionella Dot/Icm type IV secretion system. Research Abstract Details 

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Identification of the core transmembrane complex of the Legionella Dot/Icm type IV secretion system. Abstract Text:

Carr D Vincent,Jonathan R Friedman,Kwang Cheol Jeong,Emily C Buford,Jennifer L Miller,Joseph P Vogel,

Type IV secretion systems (T4SS) are utilized by a wide range of Gram negative bacteria to deliver protein and DNA substrates to recipient cells. The best characterized T4SS are the type IVA systems, which exhibit extensive similarity to the Agrobacterium VirB T4SS. In contrast, type IVB secretion systems share almost no sequence homology to the type IVA systems, are composed of approximately twice as many proteins, and remain largely uncharacterized. Type IVB systems include the Dot/Icm systems found in the pathogens Legionella and Coxiella and the conjugative apparatus of IncI plasmids. Here we report the first extensive characterization of a type IVB system, the Legionella Dot/Icm secretion apparatus. Based on biochemical and genetic analysis, we discerned the existence of a critical five-protein subassembly that spans both bacterial membranes and comprises the core of the secretion complex. This transmembrane connection is mediated by protein dimer pairs consisting of two inner membrane proteins, DotF and DotG, which are able to independently associate with DotH/DotC/DotD in the outer membrane. The Legionella core subcomplex appears to be functionally analogous to the Agrobacterium VirB7-10 subcomplex, suggesting a remarkable conservation of the core subassembly in these evolutionarily distant type IV secretion machines.

Identification of the core transmembrane complex of the Legionella Dot/Icm type IV secretion system. Publishing Authors By Initials

CD Vincent,JR Friedman,KC Jeong,EC Buford,JL Miller,JP Vogel,

For similar bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: legionellaceae: legionella: legionella pneumophila research abstracts see: bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: legionellaceae: legionella: legionella pneumophila research

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Identification of the core transmembrane complex of the Legionella Dot/Icm type IV secretion system. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular microbiology

VOLUME: 62

Page Numbers: 1278-91

Journal Abbreviation: Mol. Microbiol.

ISSN: 0950-382X

DAY: 16

MONTH: 10

YEAR: 2006

Identification of the core transmembrane complex of the Legionella Dot/Icm type IV secretion system. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8712028

Identification of the core transmembrane complex of the Legionella Dot/Icm type IV secretion system. Keywords Mesh Terms:

KEYWORDS: Legionella pneumophila

MESH TERMS: physiology

Chemical & Substance for Abstract: Identification of the core transmembrane complex of the Legionella Dot/Icm type IV secretion system. Information

Substance Name: Bacterial Proteins

Registry Number: 0

Grant and Affiliation Information for Identification of the core transmembrane complex of the Legionella Dot/Icm type IV secretion system.

AFFILIATION: Department of Molecular Microbiology, Washington University, St. Louis, MO 63110, USA.

Country: England

AGENCY: United States NIAID

GRANT: AI48052

ACRONYM: AI

MEDLINETA: Mol Microbiol

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