Identification of the amino acid residues critical for specific binding of the bacteriolytic enzyme of gamma-phage, PlyG, to Bacillus anthracis.

Identification of the amino acid residues critical for specific binding of the bacteriolytic enzyme of gamma-phage, PlyG, to Bacillus anthracis. Research Abstract Details 

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Identification of the amino acid residues critical for specific binding of the bacteriolytic enzyme of gamma-phage, PlyG, to Bacillus anthracis. Abstract Text:

Hitomi Kikkawa,Yoshihito Fujinami,Shin-ichi Suzuki,Jiro Yasuda,Hitomi Kikkawa,Yoshihito Fujinami,Shin-ichi Suzuki,Jiro Yasuda,Hitomi Kikkawa,Yoshihito Fujinami,Shin-ichi Suzuki,Jiro Yasuda,

Bacillus anthracis causes anthrax, a lethal disease affecting humans, which has attracted attention due to its bioterrorism potential. gamma-Phage specifically infects B. anthracis, and is used for its detection. gamma-Phage lysin, PlyG, specifically lyses B. anthracis. Mutational analysis of PlyGB (PlyG binding domain; residues 156-233) indicated that positions 190-199 are necessary for binding to B. anthracis. This region is the central part of PlyGB and is predicted to form a beta-sheet. The amino acid residues of this region are also conserved in other lysins specific for B. anthracis. Alanine substitution at position 190 or 199 within this region resulted in significantly reduced binding, suggesting that L190 and Q199 play key roles in binding of PlyGB to B. anthracis. Our observations provide new insight into the mechanism of specific binding of lysin to B. anthracis, and may be useful in establishing new methods for detection of B. anthracis.

Identification of the amino acid residues critical for specific binding of the bacteriolytic enzyme of gamma-phage, PlyG, to Bacillus anthracis. Publishing Authors By Initials

H Kikkawa,Y Fujinami,S Suzuki,J Yasuda,H Kikkawa,Y Fujinami,S Suzuki,J Yasuda,H Kikkawa,Y Fujinami,S Suzuki,J Yasuda,

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Identification of the amino acid residues critical for specific binding of the bacteriolytic enzyme of gamma-phage, PlyG, to Bacillus anthracis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochemical and biophysical research communication

VOLUME: 363

Page Numbers: 531-5

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 0006-291X

DAY: 11

MONTH: 09

YEAR: 2007

Identification of the amino acid residues critical for specific binding of the bacteriolytic enzyme of gamma-phage, PlyG, to Bacillus anthracis. Information

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LANGUAGE: eng

NlmUniqueID: 372516

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Grant and Affiliation Information for Identification of the amino acid residues critical for specific binding of the bacteriolytic enzyme of gamma-phage, PlyG, to Bacillus anthracis.

AFFILIATION: Third Department of Forensic Science, National Research Institute of Police Science, Kashiwa 277-0882, Japan.

Country: United States

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MEDLINETA: Biochem Biophys Res Commun

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