Identification of SNPs and development of allele-specific PCR markers for gamma-gliadin alleles in Triticum aestivum.

Identification of SNPs and development of allele-specific PCR markers for gamma-gliadin alleles in Triticum aestivum. Research Abstract Details 

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Identification of SNPs and development of allele-specific PCR markers for gamma-gliadin alleles in Triticum aestivum. Abstract Text:

W Zhang,M C Gianibelli,W Ma,L Rampling,K R Gale,

The coding regions of 28 entries of hexaploid wheat gamma-gliadin genes, gene fragments or pseudogenes in GenBank were used for nucleotide alignment. These sequences could be divided into nine subgroups based on nucleotide variation. The chromosomal locations of five of the seven unassigned subgroups were identified through subgroup-specific polymerase chain reactions (PCR) using Chinese Spring group-1 nulli-tetrasomic lines. Multiple single nucleotide polymorphisms (SNPs) and small insertions/deletions were identified in each subgroup. With further mining from wheat expressed sequence tag databases and targeted DNA sequencing, two SNPs were confirmed and one SNP was discovered for genes at the Gli-A1, Gli-B1 and Gli-D1 loci. A modified allele-specific PCR procedure for assaying SNPs was used to generate dominant DNA markers based on these three SNPs. For each of these three SNPs, two allele-specific primer sets were used to test Chinese Spring and 52 commercial Australian wheat varieties representing a range of low-molecular-weight (LMW) alleles. PCR results indicated that all were positive with one of the primer sets and negative with the other, with the exception of three varieties containing the 1BL/1RS chromosomal translocation that were negative for both. Furthermore, markers GliA1.1, GliB1.1 and GliD1.1 were found to be correlated with Glu-A3 a, b or c, Glu-B3 b, c, d or e and Glu-D3 a, b or e LMW glutenin alleles, respectively. Markers GliA1.2, GliB1.2 and GliD1.2 were found to be correlated with the Glu-A3 d or e, Glu-B3 a, g or h and Glu-D3 c alleles, respectively. These results indicated that the gamma-gliadin SNP markers could be used for detecting linked LMW glutenin subunit alleles that are important in determining the quality attributes of wheat products.

Identification of SNPs and development of allele-specific PCR markers for gamma-gliadin alleles in Triticum aestivum. Publishing Authors By Initials

W Zhang,MC Gianibelli,W Ma,L Rampling,KR Gale,

For similar plants: plant families and groups: angiosperms: poaceae: triticum research abstracts see: plants: plant families and groups: angiosperms: poaceae: triticum research

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Identification of SNPs and development of allele-specific PCR markers for gamma-gliadin alleles in Triticum aestivum. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: TAG. Theoretical and applied genetics. Theoretisch

VOLUME: 107

Page Numbers: 130-8

Journal Abbreviation:

ISSN: 0040-5752

DAY: 24

MONTH: 04

YEAR: 2003

Identification of SNPs and development of allele-specific PCR markers for gamma-gliadin alleles in Triticum aestivum. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 145600

Identification of SNPs and development of allele-specific PCR markers for gamma-gliadin alleles in Triticum aestivum. Keywords Mesh Terms:

KEYWORDS: Triticum

MESH TERMS: genetics

Chemical & Substance for Abstract: Identification of SNPs and development of allele-specific PCR markers for gamma-gliadin alleles in Triticum aestivum. Information

Substance Name: Gliadin

Registry Number: 9007-90-3

Grant and Affiliation Information for Identification of SNPs and development of allele-specific PCR markers for gamma-gliadin alleles in Triticum aestivum.

AFFILIATION: Commonwealth Scientific and Industrial Research Organisation, Plant Industry, GPO Box 1600, Canberra ACT 2601, Australia.

Country: Germany

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MEDLINETA: Theor Appl Genet

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