Identification of secondary structure elements in intermediate-resolution density maps.

Identification of secondary structure elements in intermediate-resolution density maps. Research Abstract Details 

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Identification of secondary structure elements in intermediate-resolution density maps. Abstract Text:

Matthew L Baker,Tao Ju,Wah Chiu,

An increasing number of structural studies of large macromolecular complexes, both in X-ray crystallography and cryo-electron microscopy, have resulted in intermediate-resolution (5-10 A) density maps. Despite being limited in resolution, significant structural and functional information may be extractable from these maps. To aid in the analysis and annotation of these complexes, we have developed SSEhunter, a tool for the quantitative detection of alpha helices and beta sheets. Based on density skeletonization, local geometry calculations, and a template-based search, SSEhunter has been tested and validated on a variety of simulated and authentic subnanometer-resolution density maps. The result is a robust, user-friendly approach that allows users to quickly visualize, assess, and annotate intermediate-resolution density maps. Beyond secondary structure element identification, the skeletonization algorithm in SSEhunter provides secondary structure topology, which is potentially useful in leading to structural models of individual molecular components directly from the density.

Identification of secondary structure elements in intermediate-resolution density maps. Publishing Authors By Initials

ML Baker,T Ju,W Chiu,

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Identification of secondary structure elements in intermediate-resolution density maps. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Structure (London, England : 1993)

VOLUME: 15

Page Numbers: 7-19

Journal Abbreviation: Structure

ISSN: 0969-2126

DAY: 3

MONTH: Jan

YEAR: 2007

Identification of secondary structure elements in intermediate-resolution density maps. Information

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LANGUAGE: eng

NlmUniqueID: 101087697

Identification of secondary structure elements in intermediate-resolution density maps. Keywords Mesh Terms:

KEYWORDS: Software

MESH TERMS: chemistry

Chemical & Substance for Abstract: Identification of secondary structure elements in intermediate-resolution density maps. Information

Substance Name: Proteins

Registry Number: 0

Grant and Affiliation Information for Identification of secondary structure elements in intermediate-resolution density maps.

AFFILIATION: National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA.

Country: United States

AGENCY: United States NCRR

GRANT: P41RR02250

ACRONYM: RR

MEDLINETA: Structure

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