Identification of Rad51 regulation by BRCA2 using Caenorhabditis elegans BRCA2 and bimolecular fluorescence complementation analysis.

Identification of Rad51 regulation by BRCA2 using Caenorhabditis elegans BRCA2 and bimolecular fluorescence complementation analysis. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Identification of Rad51 regulation by BRCA2 using Caenorhabditis elegans BRCA2 and bimolecular fluorescence complementation analysis. Abstract Text:

Jaewon Min,Pil-gu Park,Eunkyong Ko,Eunhee Choi,Hyunsook Lee,Jaewon Min,Pil-gu Park,Eunkyong Ko,Eunhee Choi,Hyunsook Lee,

BRCA2 is involved in double-stranded DNA break repair by binding and regulating Rad51-mediated homologous recombination. Insights as to how BRCA2 regulates Rad51-mediated DNA repair arose from in vitro biochemical studies on fragments of BRCA2. However, the large 400-kDa BRCA2 protein has hampered our ability to understand the entire process by which full-length BRCA2 regulates Rad51. Here, we show that CeBRC-2, which is only one tenth the size of mammalian BRCA2, complemented BRCA2-deficiency in Rad51 regulation. CeBRC-2 was able to bind to mammalian Rad51 (mRad51) and form distinct nuclear foci when they interacted. In our bimolecular fluorescence complementation analysis (BiFC), we show that the strength of the interaction between CeBRC-2 and mRad51 increased markedly after DNA damage. The BRC motif of CeBRC-2 was responsible for binding mRad51, but without the OB fold, the complex was unable to target damaged DNA. When CeBRC-2 was introduced into BRCA2-deficient cells, it restored Rad51 foci after DNA damage. Our study suggests a mode of action for BRCA2 with regard to DNA repair.

Identification of Rad51 regulation by BRCA2 using Caenorhabditis elegans BRCA2 and bimolecular fluorescence complementation analysis. Publishing Authors By Initials

J Min,PG Park,E Ko,E Choi,H Lee,J Min,PG Park,E Ko,E Choi,H Lee,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Identification of Rad51 regulation by BRCA2 using Caenorhabditis elegans BRCA2 and bimolecular fluorescence complementation analysis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochemical and biophysical research communication

VOLUME: 362

Page Numbers: 958-64

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 0006-291X

DAY: 27

MONTH: 08

YEAR: 2007

Identification of Rad51 regulation by BRCA2 using Caenorhabditis elegans BRCA2 and bimolecular fluorescence complementation analysis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 372516

Identification of Rad51 regulation by BRCA2 using Caenorhabditis elegans BRCA2 and bimolecular fluorescence complementation analysis. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Identification of Rad51 regulation by BRCA2 using Caenorhabditis elegans BRCA2 and bimolecular fluorescence complementation analysis. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Identification of Rad51 regulation by BRCA2 using Caenorhabditis elegans BRCA2 and bimolecular fluorescence complementation analysis.

AFFILIATION: School of Biological Sciences and Research Center for Functional Cellulomics, College of Natural Sciences, Seoul National University, San 56-1, Shillin-Dong, Gwanak-Ku, Seoul 151-742, South Korea.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Biochem Biophys Res Commun

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Identification of Rad51 regulation by BRCA2 using Caenorhabditis elegans BRCA2 and bimolecular fluorescence complementation analysis Related Publications

• A differential response of two putative mammalian circadian regulators, mper1 and mper2, to light.
• Actual Conditions and Countermeasure of Yun-bian Old Men's Health.
• Aspirin changes the secretion rate and amino acid composition of human small intestinal mucin in subjects with ileal conduits.
• Bone morphogenetic protein-2 facilitates expression of chondrogenic, not osteogenic, phenotype of human intervertebral disc cells.
• Channel architecture in maltoporin: dominance studies with lamB mutations influencing maltodextrin binding provide evidence for independent selectivity filters in each subunit.
• Compensation. Costly to a fault.
• Design and feasibility of quick EXAFS scans for a 'broomstick' double-crystal monochromator at PLS beamline.
• Directed evolution of the lambda receptor of Escherichia coli through affinity chromatographic selection.
• Effects of dietary vitamin E supplementation on lipid oxidation and volatiles content of irradiated, cooked turkey meat patties with different packaging.
• Exclusion of high-molecular-weight maltosaccharides by lipopolysaccharide O-antigen of Escherichia coli and Salmonella typhimurium.
• Family decision making for elderly patients in the intensive care unit.
• Identification of an osteogenic protein-1 responsive element in the aggrecan promoter.
• Impairment of conjunctival glutathione secretion and ion transport by oxidative stress in an adenovirus type 5 ocular infection model of pigmented rabbits.
• Inactivation of the PTEN gene protein product is associated with the invasiveness and metastasis, but not angiogenesis, of breast cancer.
• Isolation, by affinity chromatography, of mutant escherichia coli cells with novel regulation of lamB expression.
• Longitudinal clivus fracture associated with trapped basilar artery: unusual survival with good neurological recovery.
• MPer1 and mper2 are essential for normal resetting of the circadian clock.
• Model construction on quality of family caregiving in the elderly.
• Nonredundant roles of the mPer1 and mPer2 genes in the mammalian circadian clock.
• Photoionization studies of sulfur radicals and products of their reactions.
• Posterior transarticular screw fixation for chronic atlanto-axial instability.
• Reciprocal regulation of haem biosynthesis and the circadian clock in mammals.
• Restoration of a loose implant supported crown: a case report.
• Significance of chronic epilepsy in glial tumors and correlation with surgical strategies.
• The circadian gene Period2 plays an important role in tumor suppression and DNA damage response in vivo.
• The effects of permeation enhancers on the surface morphology of the rat nasal mucosa: a scanning electron microscopy study.
• The influence of maltoporin affinity on the transport of maltose and maltohexaose into Escherichia coli.
• Tissue-specific expression, heat inducibility, and biological roles of two hsp16 genes in Caenorhabditis elegans.
• Up-regulation of MUC5AC and MUC5B mucin genes in chronic rhinosinusitis.
• [Expression of MUC3, MUC5AC, MUC6 and epidermal growth factor receptor in gallbladder epithelium according to gallstone composition]