Identification of Pseudozyma graminicola CBS 10092 as a Producer of Glycolipid Biosurfactants, Mannosylerythritol Lipids.

Identification of Pseudozyma graminicola CBS 10092 as a Producer of Glycolipid Biosurfactants, Mannosylerythritol Lipids. Research Abstract Details 

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Identification of Pseudozyma graminicola CBS 10092 as a Producer of Glycolipid Biosurfactants, Mannosylerythritol Lipids. Abstract Text:

Tomotake Morita,Masaaki Konishi,Tokuma Fukuoka,Tomohiro Imura,Shuhei Yamamoto,Masaru Kitagawa,Atsushi Sogabe,Dai Kitamoto,Tomotake Morita,Masaaki Konishi,Tokuma Fukuoka,Tomohiro Imura,Shuhei Yamamoto,Masaru Kitagawa,Atsushi Sogabe,Dai Kitamoto,Tomotake Morita,Masaaki Konishi,Tokuma Fukuoka,Tomohiro Imura,Shuhei Yamamoto,Masaru Kitagawa,Atsushi Sogabe,Dai Kitamoto,Tomotake Morita,Masaaki Konishi,Tokuma Fukuoka,Tomohiro Imura,Shuhei Yamamoto,Masaru Kitagawa,Atsushi Sogabe,Dai Kitamoto,

A basidiomycetous yeast, Pseudozyma graminicola CBS 10092, was found to accumulate a large amount of glycolipids in the cultured medium when grown on soybean oil as the sole carbon source. Based on thin layer chromatography, the extracellular glycolipids gave spots corresponding to those of mannosylerythritol lipids (MELs), which are highly functional and promising biosurfactants. From the structural characterization by (1)H and (13)C NMR, the main product was identified as 4-O-[(4'-mono-O-acetyl-2', 3'-di-O-alka(e)noyl)-beta-D-mannopyranosyl]-D-erythritol, which is a highly hydrophilic derivative of MELs known as MEL-C. According to high-performance liquid chromatography analysis, the main product, MEL-C, comprised approximately 85% of all the MELs, and the total amount reached approximately 10 g/L for 7 days. The fatty acids of the present MEL-C consisted of mainly C(6), C(8) and C(14) acids, considerably different from those of MEL-C produced by other Pseudozyma strains such as P. antarctica and P. shanxiensis. The observed critical micelle concentration (CMC) and the surface-tension at CMC of the MEL-C were 4.0 x 10(-6) M and 24.2 mN/m, respectively, while those of MEL-A, the most intensively studied MEL, were 2.7 x 10(-6) M and 28.4 mN/m, respectively. This implied that the MEL-C has higher hydrophilicity than conventional MELs hitherto reported. In addition, on a water-penetration scan, the MEL-C efficiently formed the lamella phase (L(alpha)) at a wide range of concentrations, indicating its excellent self-assembling properties. From these results, the newly identified MELs produced by P. graminicola are likely to have great potential for use in oil-in-water type emulsifiers and/or washing detergents, and would thus facilitate a broad range of applications for the promising yeast biosurfactants.

Identification of Pseudozyma graminicola CBS 10092 as a Producer of Glycolipid Biosurfactants, Mannosylerythritol Lipids. Publishing Authors By Initials

T Morita,M Konishi,T Fukuoka,T Imura,S Yamamoto,M Kitagawa,A Sogabe,D Kitamoto,T Morita,M Konishi,T Fukuoka,T Imura,S Yamamoto,M Kitagawa,A Sogabe,D Kitamoto,T Morita,M Konishi,T Fukuoka,T Imura,S Yamamoto,M Kitagawa,A Sogabe,D Kitamoto,T Morita,M Konishi,T Fukuoka,T Imura,S Yamamoto,M Kitagawa,A Sogabe,D Kitamoto,

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Identification of Pseudozyma graminicola CBS 10092 as a Producer of Glycolipid Biosurfactants, Mannosylerythritol Lipids. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of oleo science

VOLUME: 57

Page Numbers: 123-31

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ISSN: 1347-3352

DAY: 16

MONTH: 01

YEAR: 2008

Identification of Pseudozyma graminicola CBS 10092 as a Producer of Glycolipid Biosurfactants, Mannosylerythritol Lipids. Information

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LANGUAGE: eng

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AFFILIATION: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST).

Country: Japan

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MEDLINETA: J Oleo Sci

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