Identification of PhIL1, a novel cytoskeletal protein of the Toxoplasma gondii pellicle, through photosensitized labeling with 5-[125I]iodonaphthalene-1-azide.

Identification of PhIL1, a novel cytoskeletal protein of the Toxoplasma gondii pellicle, through photosensitized labeling with 5-[125I]iodonaphthalene-1-azide. Research Abstract Details 

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Identification of PhIL1, a novel cytoskeletal protein of the Toxoplasma gondii pellicle, through photosensitized labeling with 5-[125I]iodonaphthalene-1-azide. Abstract Text:

Stacey D Gilk,Yossef Raviv,Ke Hu,John M Murray,Con J M Beckers,Gary E Ward,

The pellicle of the protozoan parasite Toxoplasma gondii is a unique triple bilayer structure, consisting of the plasma membrane and two tightly apposed membranes of the underlying inner membrane complex. Integral membrane proteins of the pellicle are likely to play critical roles in host cell recognition, attachment, and invasion, but few such proteins have been identified. This is in large part because the parasite surface is dominated by a family of abundant and highly immunogenic glycosylphosphatidylinositol (GPI)-anchored proteins, which has made the identification of non-GPI-linked proteins difficult. To identify such proteins, we have developed a radiolabeling approach using the hydrophobic, photoactivatable compound 5-[(125)I]iodonaphthalene-1-azide (INA). INA can be activated by photosensitizing fluorochromes; by restricting these fluorochromes to the pellicle, [(125)I]INA labeling will selectively target non-GPI-anchored membrane-embedded proteins of the pellicle. We demonstrate here that three known membrane proteins of the pellicle can indeed be labeled by photosensitization with INA. In addition, this approach has identified a novel 22-kDa protein, named PhIL1 (photosensitized INA-labeled protein 1), with unexpected properties. While the INA labeling of PhIL1 is consistent with an integral membrane protein, the protein has neither a transmembrane domain nor predicted sites of lipid modification. PhIL1 is conserved in apicomplexan parasites and localizes to the parasite periphery, concentrated at the apical end just basal to the conoid. Detergent extraction and immunolocalization data suggest that PhIL1 associates with the parasite cytoskeleton.

Identification of PhIL1, a novel cytoskeletal protein of the Toxoplasma gondii pellicle, through photosensitized labeling with 5-[125I]iodonaphthalene-1-azide. Publishing Authors By Initials

SD Gilk,Y Raviv,K Hu,JM Murray,CJ Beckers,GE Ward,

For similar animals: invertebrates: protozoa: apicomplexa: coccidia: eimeriida: sarcocystidae: toxoplasma research abstracts see: animals: invertebrates: protozoa: apicomplexa: coccidia: eimeriida: sarcocystidae: toxoplasma research

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Identification of PhIL1, a novel cytoskeletal protein of the Toxoplasma gondii pellicle, through photosensitized labeling with 5-[125I]iodonaphthalene-1-azide. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Eukaryotic cell

VOLUME: 5

Page Numbers: 1622-34

Journal Abbreviation: Eukaryotic Cell

ISSN: 1535-9778

DAY: 3

MONTH: Oct

YEAR: 2006

Identification of PhIL1, a novel cytoskeletal protein of the Toxoplasma gondii pellicle, through photosensitized labeling with 5-[125I]iodonaphthalene-1-azide. Information

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LANGUAGE: eng

NlmUniqueID: 101130731

Identification of PhIL1, a novel cytoskeletal protein of the Toxoplasma gondii pellicle, through photosensitized labeling with 5-[125I]iodonaphthalene-1-azide. Keywords Mesh Terms:

KEYWORDS: Toxoplasma

MESH TERMS: ultrastructure

Chemical & Substance for Abstract: Identification of PhIL1, a novel cytoskeletal protein of the Toxoplasma gondii pellicle, through photosensitized labeling with 5-[125I]iodonaphthalene-1-azide. Information

Substance Name: iodonaphthylazide

Registry Number: 63785-44-4

Grant and Affiliation Information for Identification of PhIL1, a novel cytoskeletal protein of the Toxoplasma gondii pellicle, through photosensitized labeling with 5-[125I]iodonaphthalene-1-azide.

AFFILIATION: Department of Microbiology and Molecular Genetics, 316 Stafford Hall, University of Vermont, Burlington, VT 05405, USA.

Country: United States

AGENCY: United States NCI

GRANT: P30 CA22435

ACRONYM: CA

MEDLINETA: Eukaryot Cell

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