Identification of nuclear proteins that interact with platinum-modified DNA by photoaffinity labeling.

Identification of nuclear proteins that interact with platinum-modified DNA by photoaffinity labeling. Research Abstract Details 

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Identification of nuclear proteins that interact with platinum-modified DNA by photoaffinity labeling. Abstract Text:

Christiana Xin Zhang,Pamela V Chang,Stephen J Lippard,

Interactions between cellular proteins and cisplatin-modified DNA are important in determining the anticancer activity of the drug. To develop a general approach for identifying proteins that mediate cellular responses to cisplatin, photoreactive cisplatin analogues having a tethered benzophenone moiety were prepared and used to form the major 1,2-intrastrand platinum-DNA cross-links. Upon irradiation of the platinated DNA dissolved in a HeLa nuclear extract, the appended photolabile benzophenone group generates a highly reactive species that binds irreversibly to cellular proteins that interact with the probe. Several DNA-protein cross-linked adducts were identified that may function in the cellular processing of cisplatin-DNA adducts. Of these, PARP-1 had not previously been demonstrated directly to contact Pt-DNA cross-links in human cells.

Identification of nuclear proteins that interact with platinum-modified DNA by photoaffinity labeling. Publishing Authors By Initials

CX Zhang,PV Chang,SJ Lippard,

For similar enzymes and coenzymes: enzymes: transferases: glycosyltransferases: pentosyltransferases: adp ribose transferases: poly(adp-ribose) polymerases research abstracts see: enzymes and coenzymes: enzymes: transferases: glycosyltransferases: pentosyltransferases: adp ribose transferases: poly(adp-ribose) polymerases research

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Identification of nuclear proteins that interact with platinum-modified DNA by photoaffinity labeling. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 126

Page Numbers: 6536-7

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 2

MONTH: Jun

YEAR: 2004

Identification of nuclear proteins that interact with platinum-modified DNA by photoaffinity labeling. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

Identification of nuclear proteins that interact with platinum-modified DNA by photoaffinity labeling. Keywords Mesh Terms:

KEYWORDS: Poly(ADP-ribose) Polymerases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Identification of nuclear proteins that interact with platinum-modified DNA by photoaffinity labeling. Information

Substance Name: Poly(ADP-ribose) Polymerases

Registry Number: EC 2.4.2.30

Grant and Affiliation Information for Identification of nuclear proteins that interact with platinum-modified DNA by photoaffinity labeling.

AFFILIATION: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Country: United States

AGENCY: United States NCI

GRANT: CA34992

ACRONYM: CA

MEDLINETA: J Am Chem Soc

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