Identification of nondisulfided pro alpha 1 (IV) chain produced by cultured B16 melanoma cells.

Identification of nondisulfided pro alpha 1 (IV) chain produced by cultured B16 melanoma cells. Research Abstract Details 

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Identification of nondisulfided pro alpha 1 (IV) chain produced by cultured B16 melanoma cells. Abstract Text:

I Tokimitsu,M Takehana,H Hori,Y Nagai,S Tajima,

Two collagenous polypeptides with apparent molecular weights of 180 and 500 kDa under a nonreducing condition were found to be produced in the culture medium of B16 mouse melanoma cells. Under a reducing condition, the higher molecular weight polypeptide (500 kDa) migrated at an identical position to the 180 kDa polypeptide on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Both polypeptides were found to have an identical primary structure by V8 protease peptide mapping. Cyanogen bromide cleavage patterns, the fragments produced by pepsin digestion, and partial amino acid sequences of the 180 kDa polypeptide were compatible with those of pro alpha 1 (IV) chain. These results indicate that B16 mouse melanoma cells preferentially produce pro alpha 1 (IV) chains consisting of two forms: disulfide- and nondisulfide-bonded forms.

Identification of nondisulfided pro alpha 1 (IV) chain produced by cultured B16 melanoma cells. Publishing Authors By Initials

I Tokimitsu,M Takehana,H Hori,Y Nagai,S Tajima,

For similar cells: cells, cultured: tumor cells, cultured research abstracts see: cells: cells, cultured: tumor cells, cultured research

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Identification of nondisulfided pro alpha 1 (IV) chain produced by cultured B16 melanoma cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 116

Page Numbers: 1039-43

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1994

Identification of nondisulfided pro alpha 1 (IV) chain produced by cultured B16 melanoma cells. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Identification of nondisulfided pro alpha 1 (IV) chain produced by cultured B16 melanoma cells. Keywords Mesh Terms:

KEYWORDS: Tumor Cells, Cultured

MESH TERMS: metabolism

Chemical & Substance for Abstract: Identification of nondisulfided pro alpha 1 (IV) chain produced by cultured B16 melanoma cells. Information

Substance Name: Collagen

Registry Number: 9007-34-5

Grant and Affiliation Information for Identification of nondisulfided pro alpha 1 (IV) chain produced by cultured B16 melanoma cells.

AFFILIATION: Department of Dermatology, Keio University School of Medicine, Tokyo.

Country: JAPAN

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MEDLINETA: J Biochem

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