Identification of neighboring protein pairs cross-linked with dimethyl 3,3'-dithiobispropionimidate in rat liver 40S ribosomal subunits.

Identification of neighboring protein pairs cross-linked with dimethyl 3,3'-dithiobispropionimidate in rat liver 40S ribosomal subunits. Research Abstract Details 

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Identification of neighboring protein pairs cross-linked with dimethyl 3,3'-dithiobispropionimidate in rat liver 40S ribosomal subunits. Abstract Text:

T Uchiumi,K Terao,K Ogata,

Rat liver 40S ribosomal subunits were treated with a bifunctional imidoester, dimethyl 3,3'-dithiobispropionimidate (DTP), and the neighboring protein pairs were identified. The cross-linked proteins were analyzed by acrylamide/SDS diagonal gel electrophoresis (Sommer & Traut (1974) Proc. Natl. Acad. Sci. U.S. 71, 3946-3950). The cross-linked components that fell off the diagonal upon adding 2-mercaptoethanol in the second dimension were labeled with 125I in the acrylamide gel and identified by two-dimensional acrylamide/urea gel electrophoresis, followed by radioautography. Considering these results and the molecular weights, we propose the following ten pairs, according to our numbering system (Terao & Ogata (1975) Biochim. Biophys. Acta 402, 219-229): S3-S5 (S3/S3a-S4), S3-S14 (S3/S3a-S14), S3-S17 (S3/S3a-S16), S5-S22 (S4-S23/S24), S10-S12 (S8-S11), S9-S16 (S9-S18), S9-S22 (S9-S23/S24), S6-S23 (S5-S25), S17-S21 (S16-S19), and S16-S26 (S18-S27). The designation according to the proposed uniform nomenclature (McConkey et al. (1979) Mol. Gen. Genet. 169, 1-6) are given in parentheses.

Identification of neighboring protein pairs cross-linked with dimethyl 3,3'-dithiobispropionimidate in rat liver 40S ribosomal subunits. Publishing Authors By Initials

T Uchiumi,K Terao,K Ogata,

For similar cells: cellular structures: intracellular space: cytoplasm: cytoplasmic structures: organelles: ribosomes research abstracts see: cells: cellular structures: intracellular space: cytoplasm: cytoplasmic structures: organelles: ribosomes research

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Identification of neighboring protein pairs cross-linked with dimethyl 3,3'-dithiobispropionimidate in rat liver 40S ribosomal subunits. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 90

Page Numbers: 185-93

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jul

YEAR: 1981

Identification of neighboring protein pairs cross-linked with dimethyl 3,3'-dithiobispropionimidate in rat liver 40S ribosomal subunits. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Identification of neighboring protein pairs cross-linked with dimethyl 3,3'-dithiobispropionimidate in rat liver 40S ribosomal subunits. Keywords Mesh Terms:

KEYWORDS: Ribosomes

MESH TERMS: analysis

Chemical & Substance for Abstract: Identification of neighboring protein pairs cross-linked with dimethyl 3,3'-dithiobispropionimidate in rat liver 40S ribosomal subunits. Information

Substance Name: dimethyl dithiobispropionimidate

Registry Number: 59012-54-3

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Country: JAPAN

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MEDLINETA: J Biochem

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