Identification of NCAM-binding peptides promoting neurite outgrowth via a heterotrimeric G-protein-coupled pathway.

Identification of NCAM-binding peptides promoting neurite outgrowth via a heterotrimeric G-protein-coupled pathway. Research Abstract Details 

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Identification of NCAM-binding peptides promoting neurite outgrowth via a heterotrimeric G-protein-coupled pathway. Abstract Text:

Raino Kristian Hansen,Claus Christensen,Irina Korshunova,Martin Kriebel,Nadine Burkarth,Vladislav V Kiselyov,Marianne Olsen, Ostergaard,Arne Holm, Volkmer,Peter S Walmod,Vladimir Berezin,Elisabeth Bock,Raino Kristian Hansen,Claus Christensen,Irina Korshunova,Martin Kriebel,Nadine Burkarth,Vladislav V Kiselyov,Marianne Olsen, Ostergaard,Arne Holm, Volkmer,Peter S Walmod,Vladimir Berezin,Elisabeth Bock,

A combinatorial library of undecapeptides was produced and utilized for the isolation of peptide binding to the fibronectin type 3 modules (F3I-F3II) of the neural cell adhesion molecule (NCAM). The isolated peptides were sequenced and produced as dendrimers. Two of the peptides (denoted ENFIN2 and ENFIN11) were confirmed to bind to F3I-F3II of NCAM by surface plasmon resonance. The peptides induced neurite outgrowth in primary cerebellar neurons and PC12E2 cells, but had no apparent neuroprotective properties. NCAM is known to activate different intracellular pathways, including signaling through the fibroblast growth factor receptor, the Src-related non-receptor tyrosine kinase Fyn, and heterotrimeric G-proteins. Interestingly, neurite outgrowth stimulated by ENFIN2 and ENFIN11 was independent of signaling through fibroblast growth factor receptor and Fyn, but could be inhibited with pertussis toxin, an inhibitor of certain heterotrimeric G-proteins. Neurite outgrowth induced by trans-homophilic NCAM was unaffected by the peptides, whereas knockdown of NCAM completely abrogated ENFIN2- and ENFIN11-induced neuritogenesis. These observations suggest that ENFIN2 and ENFIN11 induce neurite outgrowth in an NCAM-dependent manner through G-protein-coupled signal transduction pathways. Thus, ENFIN2 and ENFIN11 may be valuable for exploring this particular type of NCAM-mediated signaling.

Identification of NCAM-binding peptides promoting neurite outgrowth via a heterotrimeric G-protein-coupled pathway. Publishing Authors By Initials

RK Hansen,C Christensen,I Korshunova,M Kriebel,N Burkarth,VV Kiselyov,M Olsen,S Ostergaard,A Holm,H Volkmer,PS Walmod,V Berezin,E Bock,RK Hansen,C Christensen,I Korshunova,M Kriebel,N Burkarth,VV Kiselyov,M Olsen,S Ostergaard,A Holm,H Volkmer,PS Walmod,V Berezin,E Bock,

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Identification of NCAM-binding peptides promoting neurite outgrowth via a heterotrimeric G-protein-coupled pathway. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of neurochemistry

VOLUME: 103

Page Numbers: 1396-407

Journal Abbreviation: J. Neurochem.

ISSN: 1471-4159

DAY: 13

MONTH: 09

YEAR: 2007

Identification of NCAM-binding peptides promoting neurite outgrowth via a heterotrimeric G-protein-coupled pathway. Information

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LANGUAGE: eng

NlmUniqueID: 2985190

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Grant and Affiliation Information for Identification of NCAM-binding peptides promoting neurite outgrowth via a heterotrimeric G-protein-coupled pathway.

AFFILIATION: Protein Laboratory, Department of Neuroscience and Pharmacology, University of Copenhagen, Copenhagen, Denmark.

Country: England

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MEDLINETA: J Neurochem

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