Identification of functional paralog shift mutations: conversion of Escherichia coli malate dehydrogenase to a lactate dehydrogenase.

Identification of functional paralog shift mutations: conversion of Escherichia coli malate dehydrogenase to a lactate dehydrogenase. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Identification of functional paralog shift mutations: conversion of Escherichia coli malate dehydrogenase to a lactate dehydrogenase. Abstract Text:

Yifeng Yin,Jack F Kirsch,Yifeng Yin,Jack F Kirsch,

Five positions in the Escherichia coli malate dehydrogenase (eMDH) sequence, which distinguish MDH from lactate dehydrogenase (LDH) activity, were identified through a combination of Venn diagrams constructed from whole genomic data and from unbiased representative sequences from terminal clades. Incorporation of the five changes in eMDH sufficed to convert the enzyme from one with (k(cat)/K(m)(pyruvate))/(k(cat)/K(m)(oxaloacetate)) = 6.1 x 10(-9) to one with that ratio = 28. The substrate specificity was thus changed by a factor of 4.6 x 10(9). The k(cat)/K(m)(pyruvate) value for the pentamutant (eMDH I12V/R81Q/M85E/G210A/V214I) is 3,500 M(-1).s(-1), which is approximately equal 1/1,000 of the values found for typical wild-type LDHs. The procedure isolates an intersection of "strong forcing sets" that should prove to be of general use in switching paralog function.

Identification of functional paralog shift mutations: conversion of Escherichia coli malate dehydrogenase to a lactate dehydrogenase. Publishing Authors By Initials

Y Yin,JF Kirsch,Y Yin,JF Kirsch,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Identification of functional paralog shift mutations: conversion of Escherichia coli malate dehydrogenase to a lactate dehydrogenase. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 17353-7

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 18

MONTH: 10

YEAR: 2007

Identification of functional paralog shift mutations: conversion of Escherichia coli malate dehydrogenase to a lactate dehydrogenase. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

Identification of functional paralog shift mutations: conversion of Escherichia coli malate dehydrogenase to a lactate dehydrogenase. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Identification of functional paralog shift mutations: conversion of Escherichia coli malate dehydrogenase to a lactate dehydrogenase. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Identification of functional paralog shift mutations: conversion of Escherichia coli malate dehydrogenase to a lactate dehydrogenase.

AFFILIATION: QB3 Institute, 572 Stanley Hall, University of California, Berkeley, CA 94720-3220, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM35393

ACRONYM: GM

MEDLINETA: Proc Natl Acad Sci U S A

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Identification of functional paralog shift mutations: conversion of Escherichia coli malate dehydrogenase to a lactate dehydrogenase Related Publications

• 18F-FDG PET for mediastinal staging of lung cancer: which SUV threshold makes sense?
• Annexin V/beta5 integrin interactions regulate apoptosis of growth plate chondrocytes.
• Cofactor-directed reversible denaturation pathways: the cofactor-stabilized Escherichia coli aspartate aminotransferase homodimer unfolds through a pathway that differs from that of the apoenzyme.
• Development of a neuroprosthesis for restoring arm and hand function via functional electrical stimulation following high cervical spinal cord injury.
• Duty hours restriction and their effect on resident education and academic departments: the American perspective.
• EMG-based Control for a C5/C6 Spinal Cord Injury Upper Extremity Neuroprosthesis.
• Effects of spinal cord injury on lower-limb passive joint moments revealed through a nonlinear viscoelastic model.
• Establishing a baseline phase behavior in magnetic resonance imaging to determine normal vs. abnormal iron content in the brain.
• Hypnosis for nausea and vomiting in cancer chemotherapy: a systematic review of the research evidence.
• Identification of functional paralog shift mutations: conversion of Escherichia coli malate dehydrogenase to a lactate dehydrogenase.
• Immunophenotypes in the circulation of patients with mild cognitive impairment.
• Increased production of inflammatory cytokines in mild cognitive impairment.
• Influence of vigilance and learning on prefrontal activation in schizophrenia.
• Iron, copper, and iron regulatory protein 2 in Alzheimer's disease and related dementias.
• Iterative approach to polyketide-type structures: stereoselective synthesis of 1,3-polyols utilizing the catalytic asymmetric Overman esterification.
• Keeping Pace With GI Technology: Knowing When to Get Out.
• Mechanisms of seizure propagation in a cortical model.
• N-nitroso-melatonin releases nitric oxide in the presence of serotonin and its derivatives.
• Oral cavity cancer.
• Percutaneous multilevel decompressive laminectomy, foraminotomy, and instrumented fusion for cervical spondylotic radiculopathy and myelopathy: assessment of feasibility and surgical technique.
• Perioperative care of patients with neuromuscular disease and dysfunction.
• Preliminary demonstration of an allelic association of the IREB2 gene with Alzheimer's disease.
• Randomized comparison of phone versus in-person BRCA1/2 predisposition genetic test result disclosure counseling.
• Recombinant expression of twelve evolutionarily diverse subfamily Ialpha aminotransferases.
• Regional dissection and determination of loosely bound and non-heme iron in the developing mouse brain.
• Sigma receptor agonists provide neuroprotection in vitro by preserving bcl-2.
• Structure and function analysis of the CMS/CIN85 protein family identifies actin-bundling properties and heterotypic-complex formation.
• Sutureless circumcision using 2-octyl cyanoacrylate (Dermabond): appraisal after 18-month experience.
• The unfolding pathway for Apo Escherichia coli aspartate aminotransferase is dependent on the choice of denaturant.
• [Not Available.]