Identification of five embryonic hemoglobins of rat and ontogeny of their constituent globins during fetal development.

Identification of five embryonic hemoglobins of rat and ontogeny of their constituent globins during fetal development. Research Abstract Details 

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Identification of five embryonic hemoglobins of rat and ontogeny of their constituent globins during fetal development. Abstract Text:

S I Iwahara,Y Abe,T Okazaki,

Hemoglobins of rats switch from an embryonic to an adult type during fetal development. However, very little is known about the structures and molecular species of hemoglobins occurring in the fetal life of rats. In the present study we isolated five embryonic hemoglobins, designated E1, E2, E3, E4, and E5, from the blood of rat fetuses on day 14 of gestation by ion exchange chromatography. Reverse-phase high performance liquid chromatography revealed that these hemoglobins each consist of two kinds of globins: E1(11 alpha:epsilon 1), E2(1 alpha: epsilon 1), E3(zetta: epsilon 1), E4(1 alpha: epsilon 3), and E5(zetta: epsilon 3), respectively. The complete amino acid sequences of the zetta, epsilon 1, and epsilon 3 globins were determined. The zetta globin showed characteristic features common in alpha-type embryonic globins of known species in that the N-terminus is blocked and the amino acid at position 38 is Gln. epsilon 1 and epsilon 3 are beta-type embryonic globins, sharing 73.7% amino acid homology. Interestingly, they are more similar to the corresponding mouse beta-type embryonic globins, y and z, respectively, than to each other, implying that these globins have evolved orthologously from common ancestral proteins. It was also shown that the zetta, epsilon 1, and epsilon 3 globins are almost completely replaced by the adult type alpha and beta globins in the blood of rat fetuses by day 18 of gestation.

Identification of five embryonic hemoglobins of rat and ontogeny of their constituent globins during fetal development. Publishing Authors By Initials

SI Iwahara,Y Abe,T Okazaki,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: sequence homology: sequence homology, amino acid research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: sequence homology: sequence homology, amino acid research

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Identification of five embryonic hemoglobins of rat and ontogeny of their constituent globins during fetal development. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 119

Page Numbers: 360-6

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1996

Identification of five embryonic hemoglobins of rat and ontogeny of their constituent globins during fetal development. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Identification of five embryonic hemoglobins of rat and ontogeny of their constituent globins during fetal development. Keywords Mesh Terms:

KEYWORDS: Sequence Homology, Amino Acid

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Identification of five embryonic hemoglobins of rat and ontogeny of their constituent globins during fetal development. Information

Substance Name: Fetal Hemoglobin

Registry Number: 9034-63-3

Grant and Affiliation Information for Identification of five embryonic hemoglobins of rat and ontogeny of their constituent globins during fetal development.

AFFILIATION: Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo.

Country: JAPAN

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MEDLINETA: J Biochem

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